Enantioseparation of tartaric acid by ligand-exchange capillary electrophoresis using contactless conductivity detection

Kód výsledku v IS VaVaI

RIV/61989592:15310/13:33147307 - isvavai.cz

Výsledek na webu

http://dx.doi.org/10.1002/jssc.201300507

DOI - Digital Object Identifier

10.1002/jssc.201300507

Jazyk výsledku

angličtina

Název v původním jazyce

Enantioseparation of tartaric acid by ligand-exchange capillary electrophoresis using contactless conductivity detection

Popis výsledku v původním jazyce

A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu(II) as a central metal ion together with l-hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl2, 14 mM trans-4-hydroxy-l-proline, and 100 mM epsilon-aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide-coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from epsilon-aminocaproic acid to l-histidine. With detection limits of about 20 M, the method was applied to the analysis of wine and grape samples; only l-tartaric acid was found.

Název v anglickém jazyce

Enantioseparation of tartaric acid by ligand-exchange capillary electrophoresis using contactless conductivity detection

Popis výsledku anglicky

A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu(II) as a central metal ion together with l-hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl2, 14 mM trans-4-hydroxy-l-proline, and 100 mM epsilon-aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide-coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from epsilon-aminocaproic acid to l-histidine. With detection limits of about 20 M, the method was applied to the analysis of wine and grape samples; only l-tartaric acid was found.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

3426-3431

Kód UT WoS článku

000325482500017

EID výsledku v databázi Scopus

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