Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10105630" target="_blank" >RIV/00216208:11310/12:10105630 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/12:43908631

Výsledek na webu

<a href="http://www.springerlink.com/content/31407v4281424368/" target="_blank" >http://www.springerlink.com/content/31407v4281424368/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11627-011-9386-z" target="_blank" >10.1007/s11627-011-9386-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)

Popis výsledku v původním jazyce

Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway ( Carum carvi L.). Microspore- and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donorplants (2 n = 20) and their anther-derived derivative plants ( n = 10, 2 n = 20, 4 n = 40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parenta

Název v anglickém jazyce

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)

Popis výsledku anglicky

Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway ( Carum carvi L.). Microspore- and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donorplants (2 n = 20) and their anther-derived derivative plants ( n = 10, 2 n = 20, 4 n = 40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parenta

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

In Vitro Cellular and Developmental Biology - Plant

ISSN

1054-5476

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

30-39

Kód UT WoS článku

000300330000005

EID výsledku v databázi Scopus

—