Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010338" target="_blank" >RIV/61989592:15310/09:00010338 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/48392952:_____/09:0R000519

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.)

Popis výsledku v původním jazyce

Protocol for anther culture in carrot (Daucus carota) was applied on caraway (Carum carvi L.), a traditional medicinal and spice cross-pollinated plant species. In vitro androgenesis in caraway was studied with the aim to produce completely homozygous inbred lines. Various induction conditions as well as the effect of genotype on in vitro androgenesis were examined. Ten breeding lines of winter caraway representing third generation of forced (artificial self-pollination were used as donor plant material. Cultured anthers produced embryogenic calli, and subsequently two types of regenerated plants were obtained, namely haploids with evident microspore origin, and diploids which may represent somatic (anther wall) regenerants or spontaneous doubled haploids. The ploidy status of regenerated plants was determined by flow cytometry. This is the first report on androgenic doubled haploid production in caraway.

Název v anglickém jazyce

Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.)

Popis výsledku anglicky

Protocol for anther culture in carrot (Daucus carota) was applied on caraway (Carum carvi L.), a traditional medicinal and spice cross-pollinated plant species. In vitro androgenesis in caraway was studied with the aim to produce completely homozygous inbred lines. Various induction conditions as well as the effect of genotype on in vitro androgenesis were examined. Ten breeding lines of winter caraway representing third generation of forced (artificial self-pollination were used as donor plant material. Cultured anthers produced embryogenic calli, and subsequently two types of regenerated plants were obtained, namely haploids with evident microspore origin, and diploids which may represent somatic (anther wall) regenerants or spontaneous doubled haploids. The ploidy status of regenerated plants was determined by flow cytometry. This is the first report on androgenic doubled haploid production in caraway.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/QF4056" target="_blank" >QF4056: Využití stávajících odrůd kmínu kořenného (Carum carvi L.) a nových metod v jeho šlechtění pro zvýšení kvalitativních a kvantitativních parametrů.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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