Catalytic Electrochemical Analysis of Platinum in Pt-DNA Adducts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126274" target="_blank" >RIV/00216208:11310/12:10126274 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/12:PU98519 RIV/00216208:11130/12:8102 RIV/00064203:_____/12:8102 RIV/62156489:43210/12:43908627

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol7/7043072.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol7/7043072.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Catalytic Electrochemical Analysis of Platinum in Pt-DNA Adducts

Popis výsledku v původním jazyce

Using of platinum based cytostatic drugs in tumour diseases treatment results in the need to detect these drugs in biological samples and to determine and study the Pt-DNA adducts, which is highly important for investigation of cisplatin-resistance developed during anti-tumour treatment. The aim of this study was not only electrochemical detection of cisplatin with the use of formazone method but also preparation of platinum-DNA complex and platinum content detection in DNA. Primarily, we optimized manual electrochemical detection using differential pulse voltammetry (DPV) for PtCl2 and cisplatin. The detection detection limits (3 S/N) were estimated as 10 pM and 30 nM for PtCl2 and cisplatin, respectively. Further, we automated the optimized method and reached 50 pM detection limit for PtCl2, but the method was proved to be versatile also for detection of cisplatin, which was verified by determination of cisplatin added cell culture medium. The method was also used to monitor the cont

Název v anglickém jazyce

Catalytic Electrochemical Analysis of Platinum in Pt-DNA Adducts

Popis výsledku anglicky

Using of platinum based cytostatic drugs in tumour diseases treatment results in the need to detect these drugs in biological samples and to determine and study the Pt-DNA adducts, which is highly important for investigation of cisplatin-resistance developed during anti-tumour treatment. The aim of this study was not only electrochemical detection of cisplatin with the use of formazone method but also preparation of platinum-DNA complex and platinum content detection in DNA. Primarily, we optimized manual electrochemical detection using differential pulse voltammetry (DPV) for PtCl2 and cisplatin. The detection detection limits (3 S/N) were estimated as 10 pM and 30 nM for PtCl2 and cisplatin, respectively. Further, we automated the optimized method and reached 50 pM detection limit for PtCl2, but the method was proved to be versatile also for detection of cisplatin, which was verified by determination of cisplatin added cell culture medium. The method was also used to monitor the cont

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

17

Strana od-do

3072-3088

Kód UT WoS článku

000304413000024

EID výsledku v databázi Scopus

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