Návrh elektrochemického biosenzoru pro studium interakcí Platina (II)-DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00019367" target="_blank" >RIV/00216224:14310/07:00019367 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/07:00107864 RIV/62157124:16270/07:00001311

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A suggestion of electrochemical biosensor for study of Platinum(II)-DNA interactions

Popis výsledku v původním jazyce

As a consequence of the employment of platinum based cytostatic drugs in tumor diseases treatment, it became necessary not only to detect them in biological samples but also to determine and study the Pt-DNA adducts, which is highly important during investigation of resistance on antitumor treatment. Thus, the main objective of this work was to study the interactions between DNA and cisplatin. Primarily, we studied changes in electrochemical behavior of cisplatin in the presence of different concentrations of sodium chloride using differential pulse voltammetry.We found out that the highest signal of cisplatin was measured in 0.75 M NaCl with detection limit of 100 pM. The current accepted opinion about platinum based cytostatics mechanism of action isthat the drugs induce their cytotoxic properties through binding to the nuclear DNA. That is why we followed by investigation of DNA interactions with cisplatin. For these purposes we have suggested a new biosensor, EDTA metallothionein

Název v anglickém jazyce

A suggestion of electrochemical biosensor for study of Platinum(II)-DNA interactions

Popis výsledku anglicky

As a consequence of the employment of platinum based cytostatic drugs in tumor diseases treatment, it became necessary not only to detect them in biological samples but also to determine and study the Pt-DNA adducts, which is highly important during investigation of resistance on antitumor treatment. Thus, the main objective of this work was to study the interactions between DNA and cisplatin. Primarily, we studied changes in electrochemical behavior of cisplatin in the presence of different concentrations of sodium chloride using differential pulse voltammetry.We found out that the highest signal of cisplatin was measured in 0.75 M NaCl with detection limit of 100 pM. The current accepted opinion about platinum based cytostatics mechanism of action isthat the drugs induce their cytotoxic properties through binding to the nuclear DNA. That is why we followed by investigation of DNA interactions with cisplatin. For these purposes we have suggested a new biosensor, EDTA metallothionein

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

2-3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

331-338

Kód UT WoS článku

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EID výsledku v databázi Scopus

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