Mechanistic study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus Ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126588" target="_blank" >RIV/00216208:11310/12:10126588 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/12:00388819

Výsledek na webu

<a href="http://pubs.acs.org/doi/pdfplus/10.1021/es3029507" target="_blank" >http://pubs.acs.org/doi/pdfplus/10.1021/es3029507</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/es3029507" target="_blank" >10.1021/es3029507</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mechanistic study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus Ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms

Popis výsledku v původním jazyce

The white rot fungus Pleurotus ostreatus is able to completely remove the synthetic hormone 17?-ethinylestradiol (EE2, 200 ?g in 20 mL) from a liquid complex or mineral medium in 3 or 14 days, respectively. Its efficiency has also been documented in theremoval of estrogenic activity that correlated with the EE2 degradation. A set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that EE2 was degraded by isolated laccase (about 90% within 24 h). The degradation was also tested with concentrated extracellular liquid where degradation reached 50% mainly due to the laccase activity; however, after a supplementation with H 2O2 and Mn2+, residual manganese-dependent peroxidase activities (40 timeslower than Lac) raised the degradation to 100%. Moreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium were found to be efficient in the degradation too. Isolated microsomal proteins appeared t

Název v anglickém jazyce

Mechanistic study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus Ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms

Popis výsledku anglicky

The white rot fungus Pleurotus ostreatus is able to completely remove the synthetic hormone 17?-ethinylestradiol (EE2, 200 ?g in 20 mL) from a liquid complex or mineral medium in 3 or 14 days, respectively. Its efficiency has also been documented in theremoval of estrogenic activity that correlated with the EE2 degradation. A set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that EE2 was degraded by isolated laccase (about 90% within 24 h). The degradation was also tested with concentrated extracellular liquid where degradation reached 50% mainly due to the laccase activity; however, after a supplementation with H 2O2 and Mn2+, residual manganese-dependent peroxidase activities (40 timeslower than Lac) raised the degradation to 100%. Moreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium were found to be efficient in the degradation too. Isolated microsomal proteins appeared t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science and Technology

ISSN

0013-936X

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

13377-13385

Kód UT WoS článku

000312432200044

EID výsledku v databázi Scopus

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