Enantioselective separation of unusual amino acids by high performance liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10139881" target="_blank" >RIV/00216208:11310/13:10139881 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.seppur.2013.09.010" target="_blank" >http://dx.doi.org/10.1016/j.seppur.2013.09.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.seppur.2013.09.010" target="_blank" >10.1016/j.seppur.2013.09.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enantioselective separation of unusual amino acids by high performance liquid chromatography

Popis výsledku v původním jazyce

Unusual amino acids, i.e. amino acids not encoded by DNA, play fundamental roles in many scientific fields. Since the single enantiomeric form can cause different and often serious response of organisms, chiral separations of unusual amino acids are irreplaceable tools in their study and their employment. Two types of chiral stationary phases, two teicoplanin-based and four polysaccharidebased columns, were used. Separation conditions of reversed phase mode, polar organic mode and hydrophilic interaction chromatography were evaluated and compared. All columns used exhibited significantly different enantioselectivities. Teicoplanin-based chiral stationary phases, especially Chirobiotic T column, were able to separate almost all enantiomers tested, withthe exception of Z-D,L- 4-F-phenylalanine ethyl ester. No partial enantioseparation of this analyte was obtained on teicoplanin- based chiral stationary phases, while baseline enantioresolution was achieved on polysaccharide- based column

Název v anglickém jazyce

Enantioselective separation of unusual amino acids by high performance liquid chromatography

Popis výsledku anglicky

Unusual amino acids, i.e. amino acids not encoded by DNA, play fundamental roles in many scientific fields. Since the single enantiomeric form can cause different and often serious response of organisms, chiral separations of unusual amino acids are irreplaceable tools in their study and their employment. Two types of chiral stationary phases, two teicoplanin-based and four polysaccharidebased columns, were used. Separation conditions of reversed phase mode, polar organic mode and hydrophilic interaction chromatography were evaluated and compared. All columns used exhibited significantly different enantioselectivities. Teicoplanin-based chiral stationary phases, especially Chirobiotic T column, were able to separate almost all enantiomers tested, withthe exception of Z-D,L- 4-F-phenylalanine ethyl ester. No partial enantioseparation of this analyte was obtained on teicoplanin- based chiral stationary phases, while baseline enantioresolution was achieved on polysaccharide- based column

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/LH11018" target="_blank" >LH11018: Cyklofruktany a jejich deriváty jako nové chirální selektory pro kapalinovou chromatografii a kapilární elektroforézu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Separation and Purification Technology

ISSN

1383-5866

e-ISSN

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Svazek periodika

119

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

123-128

Kód UT WoS článku

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EID výsledku v databázi Scopus

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