Analysis of wax esters by silver-ion high-performance liquid chromatography-tandem mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10159115" target="_blank" >RIV/00216208:11310/13:10159115 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/13:00395097

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2013.06.031" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2013.06.031</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2013.06.031" target="_blank" >10.1016/j.chroma.2013.06.031</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of wax esters by silver-ion high-performance liquid chromatography-tandem mass spectrometry

Popis výsledku v původním jazyce

Wax esters (WEs), esters of long-chain fatty acids and long-chain alcohols, were analysed by Ag-HPLC/APCI-MS/MS. Two ChromSpher Lipids columns connected in series (a total length of 50 cm) and hexane-2-propanol-acetonitrile mobile phases were used to achieve good separation of the molecular species. The chromatographic behaviour of WEs was studied under optimised conditions: retention increased with the number of double bonds and with the temperature (15-35 degrees C); retention times were affected by the double-bond position, trans isomers eluted earlier than cis isomers, and the WEs were partially separated depending on the aliphatic-chain length. The WEs provided simple APCI spectra with [M+H](+) ions, the MS/MS spectra showed fragments, which allowed their identification. The method was applied for an analysis of the WE mixtures from jojoba oil and human hair and the results were compared with analogous data from an optimised RP-HPLC system.

Název v anglickém jazyce

Analysis of wax esters by silver-ion high-performance liquid chromatography-tandem mass spectrometry

Popis výsledku anglicky

Wax esters (WEs), esters of long-chain fatty acids and long-chain alcohols, were analysed by Ag-HPLC/APCI-MS/MS. Two ChromSpher Lipids columns connected in series (a total length of 50 cm) and hexane-2-propanol-acetonitrile mobile phases were used to achieve good separation of the molecular species. The chromatographic behaviour of WEs was studied under optimised conditions: retention increased with the number of double bonds and with the temperature (15-35 degrees C); retention times were affected by the double-bond position, trans isomers eluted earlier than cis isomers, and the WEs were partially separated depending on the aliphatic-chain length. The WEs provided simple APCI spectra with [M+H](+) ions, the MS/MS spectra showed fragments, which allowed their identification. The method was applied for an analysis of the WE mixtures from jojoba oil and human hair and the results were compared with analogous data from an optimised RP-HPLC system.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F09%2F0139" target="_blank" >GA203/09/0139: Komplexní lipidomická charakterizace rostlinných a živočišných tkání</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1302

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

105-110

Kód UT WoS článku

000322349700014

EID výsledku v databázi Scopus

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