Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10144018" target="_blank" >RIV/00216208:11310/14:10144018 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2478/s11696-013-0422-9" target="_blank" >http://dx.doi.org/10.2478/s11696-013-0422-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11696-013-0422-9" target="_blank" >10.2478/s11696-013-0422-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography

Popis výsledku v původním jazyce

Fused silica capillary columns of the internal diameter of 320 mu m were packed with the Nucleosil C18 stationary phase of 5 mu m using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied tostudy their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased. (C) 2013 Institute of Chemistry, Slovak Academy of Sciences

Název v anglickém jazyce

Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography

Popis výsledku anglicky

Fused silica capillary columns of the internal diameter of 320 mu m were packed with the Nucleosil C18 stationary phase of 5 mu m using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied tostudy their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased. (C) 2013 Institute of Chemistry, Slovak Academy of Sciences

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

0366-6352

e-ISSN

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Svazek periodika

68

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

22-28

Kód UT WoS článku

000324491200003

EID výsledku v databázi Scopus

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