Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10218317" target="_blank" >RIV/00216208:11310/14:10218317 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/14:00428273

Výsledek na webu

<a href="http://dx.doi.org/10.1093/jxb/ert407" target="_blank" >http://dx.doi.org/10.1093/jxb/ert407</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/ert407" target="_blank" >10.1093/jxb/ert407</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach

Popis výsledku v původním jazyce

Chloroplast number per cell is a frequently examined quantitative anatomical parameter, often estimated by counting chloroplast profiles in two-dimensional (2D) sections of mesophyll cells. However, a mesophyll cell is a three-dimensional (3D) structureand this has to be taken into account when quantifying its internal structure. We compared 2D and 3D approaches to chloroplast counting from different points of view: (i) in practical measurements of mesophyll cells of Norway spruce needles, (ii) in a 3Dmodel of a mesophyll cell with chloroplasts, and (iii) using a theoretical analysis. We applied, for the first time, the stereological method of an optical disector based on counting chloroplasts in stacks of spruce needle optical cross-sections acquired by confocal laser-scanning microscopy. This estimate was compared with counting chloroplast profiles in 2D sections from the same stacks of sections. Comparing practical measurements of mesophyll cells, calculations performed in a 3D mo

Název v anglickém jazyce

Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach

Popis výsledku anglicky

Chloroplast number per cell is a frequently examined quantitative anatomical parameter, often estimated by counting chloroplast profiles in two-dimensional (2D) sections of mesophyll cells. However, a mesophyll cell is a three-dimensional (3D) structureand this has to be taken into account when quantifying its internal structure. We compared 2D and 3D approaches to chloroplast counting from different points of view: (i) in practical measurements of mesophyll cells of Norway spruce needles, (ii) in a 3Dmodel of a mesophyll cell with chloroplasts, and (iii) using a theoretical analysis. We applied, for the first time, the stereological method of an optical disector based on counting chloroplasts in stacks of spruce needle optical cross-sections acquired by confocal laser-scanning microscopy. This estimate was compared with counting chloroplast profiles in 2D sections from the same stacks of sections. Comparing practical measurements of mesophyll cells, calculations performed in a 3D mo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP501%2F10%2F0340" target="_blank" >GAP501/10/0340: Vliv zvýšené koncentrace CO2 a ozářenosti na strukturu a funkci fotosyntetického aparátu dřevin na různých hierarchických úrovních</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

65

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

609-620

Kód UT WoS článku

000331119300020

EID výsledku v databázi Scopus

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