Opioid-Receptor (OR) Signaling Cascades in Rat Cerebral Cortex and Model Cell Lines: the Role of Plasma Membrane Structure

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10227230" target="_blank" >RIV/00216208:11310/14:10227230 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/14:00427645 RIV/61388955:_____/14:00427645

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Opioid-Receptor (OR) Signaling Cascades in Rat Cerebral Cortex and Model Cell Lines: the Role of Plasma Membrane Structure

Popis výsledku v původním jazyce

Proteomic analysis of plasma membrane fractions (PM) izolated from brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days indicated that the brain cortex of morphine-treated animals can not be regarded as being adapted tothis drug as significant up-regulation of proteins functionally related to oxidative stress and alternation of brain energy metabolism occurred. The number of delta-OR was increased 2x and their sensitivity to monovalent cations altered. Our studies of plasma membrane structure and function in context with desensitization of GPCR action were extended by data indicating participation of cholesterol-enriched membrane domains in agonist-specific internalization of delta-OR. In HEK293 cells stably expressing delta-OR-Gi1alfa fusion protein, depletion of PM cholesterol was associated with decrease (by two-orders of magnitude) in affinity /potency of G-protein-response to agonist stimulation; maximum response was unchanged. Hydrophobic interi

Název v anglickém jazyce

Opioid-Receptor (OR) Signaling Cascades in Rat Cerebral Cortex and Model Cell Lines: the Role of Plasma Membrane Structure

Popis výsledku anglicky

Proteomic analysis of plasma membrane fractions (PM) izolated from brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days indicated that the brain cortex of morphine-treated animals can not be regarded as being adapted tothis drug as significant up-regulation of proteins functionally related to oxidative stress and alternation of brain energy metabolism occurred. The number of delta-OR was increased 2x and their sensitivity to monovalent cations altered. Our studies of plasma membrane structure and function in context with desensitization of GPCR action were extended by data indicating participation of cholesterol-enriched membrane domains in agonist-specific internalization of delta-OR. In HEK293 cells stably expressing delta-OR-Gi1alfa fusion protein, depletion of PM cholesterol was associated with decrease (by two-orders of magnitude) in affinity /potency of G-protein-response to agonist stimulation; maximum response was unchanged. Hydrophobic interi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

63

Číslo periodika v rámci svazku

Suppl. 1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

"S165"-"S176"

Kód UT WoS článku

000332073100014

EID výsledku v databázi Scopus

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