Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F14%3A00427791" target="_blank" >RIV/67985823:_____/14:00427791 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1477-5956-12-11" target="_blank" >http://dx.doi.org/10.1186/1477-5956-12-11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1477-5956-12-11" target="_blank" >10.1186/1477-5956-12-11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Popis výsledku v původním jazyce

Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from frontal brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days. In the PNS, the 10 up- or down-regulatedproteins exhibiting the largest morphine-induced change were selected, excised manually from the gel and identified by MALDI-TOF MS/MS. The identified proteins were of cytoplasmic, cell membrane, endoplasmic reticulum and mitochondrial origin and 9 of them were significantly increased. In PM, the 18 up- or down-regulated proteins were identified by LC-MS/MS and were of plasma membrane, myelin membrane, cytoplasmic and mitochondrial origin. The only member of GPCR-initiated signaling cascades identifiedin PM as altered by morphine was G? subunit. Surprisingly, the immunoblot analysis of the same PM indicated that the ?active, morphine-induced pool of G? subunits, represented just a minor fraction of the total signal of G? which was decr

Název v anglickém jazyce

Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Popis výsledku anglicky

Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from frontal brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days. In the PNS, the 10 up- or down-regulatedproteins exhibiting the largest morphine-induced change were selected, excised manually from the gel and identified by MALDI-TOF MS/MS. The identified proteins were of cytoplasmic, cell membrane, endoplasmic reticulum and mitochondrial origin and 9 of them were significantly increased. In PM, the 18 up- or down-regulated proteins were identified by LC-MS/MS and were of plasma membrane, myelin membrane, cytoplasmic and mitochondrial origin. The only member of GPCR-initiated signaling cascades identifiedin PM as altered by morphine was G? subunit. Surprisingly, the immunoblot analysis of the same PM indicated that the ?active, morphine-induced pool of G? subunits, represented just a minor fraction of the total signal of G? which was decr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proteome Science

ISSN

1477-5956

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

Feb 14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

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Kód UT WoS článku

000332800900002

EID výsledku v databázi Scopus

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