Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00461865" target="_blank" >RIV/67985823:_____/16:00461865 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jprot.2016.02.019" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2016.02.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jprot.2016.02.019" target="_blank" >10.1016/j.jprot.2016.02.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

Popis výsledku v původním jazyce

Proteomic analysis was performed in post-nuclear supernatant fraction (PNS) prepared from forebrain cortex of rats exposed to increasing doses of morphine (10–50 mg/kg) for 10 days and sacrificed 24 h (group + M10) or 20 days (group + M10/M20) after the last dose of morphine. PNS fraction was resolved by 2D-ELFO and stained by CBB. Analysis of the difference between (+ M10) and ( M10) samples of PNS by PDQuest accompanied by MALDI-TOF MS/MS indicated the significant change of 28 proteins. Importantly, the number of altered proteins was decreased to 14 after 20 days of nurturing animals in the absence of morphine. This new and important finding indicating the ability of mammalian organism to return to physiological norm after removal of the drug was verified by an independent methodology — gel-free & label-free quantification and normalization procedure denominated as MaxLFQ. The 113 proteins were identified as altered by morphine in (+ M10) samples when compared with ( M10) samples of PNS and this number was decreased to 19 after 20 days of nurturing the animals in the absence of this drug.

Název v anglickém jazyce

Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

Popis výsledku anglicky

Proteomic analysis was performed in post-nuclear supernatant fraction (PNS) prepared from forebrain cortex of rats exposed to increasing doses of morphine (10–50 mg/kg) for 10 days and sacrificed 24 h (group + M10) or 20 days (group + M10/M20) after the last dose of morphine. PNS fraction was resolved by 2D-ELFO and stained by CBB. Analysis of the difference between (+ M10) and ( M10) samples of PNS by PDQuest accompanied by MALDI-TOF MS/MS indicated the significant change of 28 proteins. Importantly, the number of altered proteins was decreased to 14 after 20 days of nurturing animals in the absence of morphine. This new and important finding indicating the ability of mammalian organism to return to physiological norm after removal of the drug was verified by an independent methodology — gel-free & label-free quantification and normalization procedure denominated as MaxLFQ. The 113 proteins were identified as altered by morphine in (+ M10) samples when compared with ( M10) samples of PNS and this number was decreased to 19 after 20 days of nurturing the animals in the absence of this drug.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Proteomics

ISSN

1874-3919

e-ISSN

—

Svazek periodika

145

Číslo periodika v rámci svazku

Aug 11

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

11-23

Kód UT WoS článku

000383931000003

EID výsledku v databázi Scopus

2-s2.0-84959499628