Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00524280" target="_blank" >RIV/67985823:_____/20:00524280 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10413205

Výsledek na webu

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231721" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231721</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0231721" target="_blank" >10.1371/journal.pone.0231721</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal

Popis výsledku v původním jazyce

Opioid addiction is recognized as a chronic relapsing brain disease resulting from repeated exposure to opioid drugs. Cellular and molecular mechanisms underlying the ability of organism to return back to the physiological norm after cessation of drug supply are not fully understood. The aim of this work was to extend our previous studies of morphine-induced alteration of rat forebrain cortex protein composition to the hippocampus. Rats were exposed to morphine for 10 days and sacrificed 24 h (groups +M10 and -M10) or 20 days after the last dose of morphine (groups +M10/-M20 and -M10/-M20). The six altered proteins (>= 2-fold) were identified in group (+M10) when compared with group (-M10) by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). The number of differentially expressed proteins was increased to thirteen after 20 days of the drug withdrawal. Noticeably, the altered level of alpha-synuclein, beta-synuclein, alpha-enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was also determined in both (+/- M10) and (+/- M10/-M20) samples of hippocampus. Immunoblot analysis of 2D gels by specific antibodies oriented against alpha/beta-synucleins and GAPDH confirmed the data obtained by 2D-DIGE analysis. Label-free quantification identified nineteen differentially expressed proteins in group (+M10) when compared with group (-M10). After 20 days of morphine withdrawal (+/- M10/-M20), the number of altered proteins was increased to twenty. We conclude that the morphine-induced alteration of protein composition in rat hippocampus after cessation of drug supply proceeds in a different manner when compared with the forebrain cortex. In forebrain cortex, the total number of altered proteins was decreased after 20 days without morphine, whilst in hippocampus, it was increased.

Název v anglickém jazyce

Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal

Popis výsledku anglicky

Opioid addiction is recognized as a chronic relapsing brain disease resulting from repeated exposure to opioid drugs. Cellular and molecular mechanisms underlying the ability of organism to return back to the physiological norm after cessation of drug supply are not fully understood. The aim of this work was to extend our previous studies of morphine-induced alteration of rat forebrain cortex protein composition to the hippocampus. Rats were exposed to morphine for 10 days and sacrificed 24 h (groups +M10 and -M10) or 20 days after the last dose of morphine (groups +M10/-M20 and -M10/-M20). The six altered proteins (>= 2-fold) were identified in group (+M10) when compared with group (-M10) by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). The number of differentially expressed proteins was increased to thirteen after 20 days of the drug withdrawal. Noticeably, the altered level of alpha-synuclein, beta-synuclein, alpha-enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was also determined in both (+/- M10) and (+/- M10/-M20) samples of hippocampus. Immunoblot analysis of 2D gels by specific antibodies oriented against alpha/beta-synucleins and GAPDH confirmed the data obtained by 2D-DIGE analysis. Label-free quantification identified nineteen differentially expressed proteins in group (+M10) when compared with group (-M10). After 20 days of morphine withdrawal (+/- M10/-M20), the number of altered proteins was increased to twenty. We conclude that the morphine-induced alteration of protein composition in rat hippocampus after cessation of drug supply proceeds in a different manner when compared with the forebrain cortex. In forebrain cortex, the total number of altered proteins was decreased after 20 days without morphine, whilst in hippocampus, it was increased.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

25

Strana od-do

e0231721

Kód UT WoS článku

000529957800001

EID výsledku v databázi Scopus

2-s2.0-85083327650