Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10283382" target="_blank" >RIV/00216208:11310/14:10283382 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/14:00441102

Výsledek na webu

<a href="http://dx.doi.org/10.1098/rsta.2014.0199" target="_blank" >http://dx.doi.org/10.1098/rsta.2014.0199</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1098/rsta.2014.0199" target="_blank" >10.1098/rsta.2014.0199</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Popis výsledku v původním jazyce

In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles,

Název v anglickém jazyce

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Popis výsledku anglicky

In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DD - Geochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP210%2F10%2F0467" target="_blank" >GAP210/10/0467: Detekce exobiologických markerů Ramanovou spektrometrií jako hlavní metodou</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

ISSN

1364-503X

e-ISSN

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Svazek periodika

372

Číslo periodika v rámci svazku

2030

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

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Kód UT WoS článku

000344991900007

EID výsledku v databázi Scopus

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