Determination of thermodynamic values of acidic dissociation constants and complexation constants of profens and their utilization for optimization of separation conditions by Simul 5 Complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10284530" target="_blank" >RIV/00216208:11310/14:10284530 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2014.08.070" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.08.070</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2014.08.070" target="_blank" >10.1016/j.chroma.2014.08.070</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of thermodynamic values of acidic dissociation constants and complexation constants of profens and their utilization for optimization of separation conditions by Simul 5 Complex

Popis výsledku v původním jazyce

In this paper we determine acid dissociation constants, limiting ionic mobilities, complexation constants with beta-cyclodextrin or heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin, and mobilities of resulting complexes of profens, using capillary zone electrophoresis and affinity capillary electrophoresis. Complexation parameters are determined for both neutral and fully charged forms of profens and further corrected for actual ionic strength and variable viscosity in order to obtain thermodynamic valuesof complexation constants. The accuracy of obtained complexation parameters is verified by multidimensional nonlinear regression of affinity capillary electrophoretic data, which provides the acid dissociation and complexation parameters within one setof measurements, and by NMR technique. A good agreement among all discussed methods was obtained. Determined complexation parameters were used as input parameters for simulations of electrophoretic separation of profens by Simul 5 Complex

Název v anglickém jazyce

Determination of thermodynamic values of acidic dissociation constants and complexation constants of profens and their utilization for optimization of separation conditions by Simul 5 Complex

Popis výsledku anglicky

In this paper we determine acid dissociation constants, limiting ionic mobilities, complexation constants with beta-cyclodextrin or heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin, and mobilities of resulting complexes of profens, using capillary zone electrophoresis and affinity capillary electrophoresis. Complexation parameters are determined for both neutral and fully charged forms of profens and further corrected for actual ionic strength and variable viscosity in order to obtain thermodynamic valuesof complexation constants. The accuracy of obtained complexation parameters is verified by multidimensional nonlinear regression of affinity capillary electrophoretic data, which provides the acid dissociation and complexation parameters within one setof measurements, and by NMR technique. A good agreement among all discussed methods was obtained. Determined complexation parameters were used as input parameters for simulations of electrophoretic separation of profens by Simul 5 Complex

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1364

Číslo periodika v rámci svazku

October 17

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

276-288

Kód UT WoS článku

000343359200031

EID výsledku v databázi Scopus

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