Preparation of silybin phase II metabolites: Streptomyces catalyzed glucuronidation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10289023" target="_blank" >RIV/00216208:11310/14:10289023 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/14:00435567

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.molcatb.2014.02.008" target="_blank" >http://dx.doi.org/10.1016/j.molcatb.2014.02.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molcatb.2014.02.008" target="_blank" >10.1016/j.molcatb.2014.02.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of silybin phase II metabolites: Streptomyces catalyzed glucuronidation

Popis výsledku v původním jazyce

Flavonolignan silybin is a major component of the silymarin complex isolated from seeds of the milk thistle (Silybum marianum) having strong antioxidant and hepatoprotective effects, and also anticancer, chemoprotective, dermatoprotective and hypocholesterolemic activities. Natural silybin (silibinin in pharmacological literature) is a mixture of two diastereomers: silybin A and silybin B. Their metabolism is strongly linked to Phase II biotransformations and respective conjugates are rapidly excreted in bile and urine. Conjugation reactions of both silybins are strictly stereoselective. Therefore, optically pure compounds must be used for metabolic studies. The aim of this study was to obtain the glucuronidated metabolites of both silybin A and B. Streptomyces sp. strain M52104 was found to be a highly effective tool for the preparation of silybin A-20-O-beta-glucuronide, silybin B-20-O-beta-glucuronide, silybin glucuronide and silybin B-7-O-beta-glucuronide and minor amounts of silyb

Název v anglickém jazyce

Preparation of silybin phase II metabolites: Streptomyces catalyzed glucuronidation

Popis výsledku anglicky

Flavonolignan silybin is a major component of the silymarin complex isolated from seeds of the milk thistle (Silybum marianum) having strong antioxidant and hepatoprotective effects, and also anticancer, chemoprotective, dermatoprotective and hypocholesterolemic activities. Natural silybin (silibinin in pharmacological literature) is a mixture of two diastereomers: silybin A and silybin B. Their metabolism is strongly linked to Phase II biotransformations and respective conjugates are rapidly excreted in bile and urine. Conjugation reactions of both silybins are strictly stereoselective. Therefore, optically pure compounds must be used for metabolic studies. The aim of this study was to obtain the glucuronidated metabolites of both silybin A and B. Streptomyces sp. strain M52104 was found to be a highly effective tool for the preparation of silybin A-20-O-beta-glucuronide, silybin B-20-O-beta-glucuronide, silybin glucuronide and silybin B-7-O-beta-glucuronide and minor amounts of silyb

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular Catalysis - B Enzymatic

ISSN

1381-1177

e-ISSN

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Svazek periodika

102

Číslo periodika v rámci svazku

April 2014

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

167-173

Kód UT WoS článku

000335872900024

EID výsledku v databázi Scopus

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