Application of Flow Cytometry for Genome Size Determination in Geosmithia Fungi: A Comparison of Methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10289248" target="_blank" >RIV/00216208:11310/14:10289248 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/14:00436051

Výsledek na webu

<a href="http://dx.doi.org/10.1002/cyto.a.22500" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22500</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.a.22500" target="_blank" >10.1002/cyto.a.22500</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of Flow Cytometry for Genome Size Determination in Geosmithia Fungi: A Comparison of Methods

Popis výsledku v původním jazyce

Genome size has played an important role in the evolution of plants and animals because changes in genome size seem to accompany if not facilitate evolutionary adaptation to environmental conditions. Flow cytometry (FCM) is a widespread method for determining genome size thanks to its high accuracy and speed of measurements. Nevertheless, only a few comparative studies of FCM methods exist in the field of mycology, and reviews are absent. In this study, we compared the suitability of several concentrations and RNAse A incubation times, fixatives and buffers for estimating genome size in fungi. We chose the genus Geosmithia as a model filamentous fungus. We also introduced a new standard, Aspergillus fumigatus CEA10, to determine absolute genome size. We found FCM to be an appropriate method for measuring genome size in fungi, but optimization steps showed that incorrect propidium iodide staining of nuclei can overestimate genome size due to cytoplasmic staining. We identified fixation

Název v anglickém jazyce

Application of Flow Cytometry for Genome Size Determination in Geosmithia Fungi: A Comparison of Methods

Popis výsledku anglicky

Genome size has played an important role in the evolution of plants and animals because changes in genome size seem to accompany if not facilitate evolutionary adaptation to environmental conditions. Flow cytometry (FCM) is a widespread method for determining genome size thanks to its high accuracy and speed of measurements. Nevertheless, only a few comparative studies of FCM methods exist in the field of mycology, and reviews are absent. In this study, we compared the suitability of several concentrations and RNAse A incubation times, fixatives and buffers for estimating genome size in fungi. We chose the genus Geosmithia as a model filamentous fungus. We also introduced a new standard, Aspergillus fumigatus CEA10, to determine absolute genome size. We found FCM to be an appropriate method for measuring genome size in fungi, but optimization steps showed that incorrect propidium iodide staining of nuclei can overestimate genome size due to cytoplasmic staining. We identified fixation

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry Part A

ISSN

1552-4922

e-ISSN

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Svazek periodika

85A

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

854-861

Kód UT WoS článku

000342636200005

EID výsledku v databázi Scopus

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