Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057231" target="_blank" >RIV/00216224:14310/12:00057231 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1469-8137.2011.03942.x" target="_blank" >http://dx.doi.org/10.1111/j.1469-8137.2011.03942.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1469-8137.2011.03942.x" target="_blank" >10.1111/j.1469-8137.2011.03942.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability

Popis výsledku v původním jazyce

Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6?47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMAdata exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39?4.09) assumed for the base-specific dye used.

Název v anglickém jazyce

Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability

Popis výsledku anglicky

Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6?47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMAdata exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39?4.09) assumed for the base-specific dye used.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

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Svazek periodika

193

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

513-521

Kód UT WoS článku

000298591800024

EID výsledku v databázi Scopus

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