Molecular and Biological Analysis of Potato virus M (PVM) Isolates from the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10296577" target="_blank" >RIV/00216208:11310/15:10296577 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/15:00451389 RIV/60109807:_____/15:#0000334

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jph.12334" target="_blank" >http://dx.doi.org/10.1111/jph.12334</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jph.12334" target="_blank" >10.1111/jph.12334</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular and Biological Analysis of Potato virus M (PVM) Isolates from the Czech Republic

Popis výsledku v původním jazyce

The sequences of the 30-terminal region of four Czech Potato virus M isolates VIRUBRA 4/007, VIRUBRA 4/009, VIRUBRA 4/016 and VIRUBRA 4/035 were determined and compared with sequences of PVM isolates available in GenBank. Among the Czech isolates, VIRUBRA 4/007 and 4/016 as well as VIRUBRA 4/016 and 4/035 showed the highest nucleotide identity (93%). Isolates VIRUBRA 4/007, 4/016 and 4/035 were most similar to the PV0273 isolate from Germany and to the wild isolate from Russia. Interestingly, isolate VIRUBRA 4/009 significantly differed from the other three Czech isolates and was the only European isolate that showed the highest nucleotide identity with American isolates. Moreover, the PVM isolates from the Czech Republic and Germany differed in theirhost range. Phylogenetic analysis based on ORF5 coding for coat protein showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on molecular and biologic

Název v anglickém jazyce

Molecular and Biological Analysis of Potato virus M (PVM) Isolates from the Czech Republic

Popis výsledku anglicky

The sequences of the 30-terminal region of four Czech Potato virus M isolates VIRUBRA 4/007, VIRUBRA 4/009, VIRUBRA 4/016 and VIRUBRA 4/035 were determined and compared with sequences of PVM isolates available in GenBank. Among the Czech isolates, VIRUBRA 4/007 and 4/016 as well as VIRUBRA 4/016 and 4/035 showed the highest nucleotide identity (93%). Isolates VIRUBRA 4/007, 4/016 and 4/035 were most similar to the PV0273 isolate from Germany and to the wild isolate from Russia. Interestingly, isolate VIRUBRA 4/009 significantly differed from the other three Czech isolates and was the only European isolate that showed the highest nucleotide identity with American isolates. Moreover, the PVM isolates from the Czech Republic and Germany differed in theirhost range. Phylogenetic analysis based on ORF5 coding for coat protein showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on molecular and biologic

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/1M06030" target="_blank" >1M06030: Funkční genomika a proteomika ve šlechtění rostlin</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology

ISSN

0931-1785

e-ISSN

—

Svazek periodika

163

Číslo periodika v rámci svazku

11-12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

1031-1035

Kód UT WoS článku

000363730100020

EID výsledku v databázi Scopus

2-s2.0-84945127730