pREPORT: a multi-readout transcription reporter vector for fission yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314875" target="_blank" >RIV/00216208:11310/15:10314875 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/yea.3055" target="_blank" >http://dx.doi.org/10.1002/yea.3055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/yea.3055" target="_blank" >10.1002/yea.3055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

pREPORT: a multi-readout transcription reporter vector for fission yeast

Popis výsledku v původním jazyce

Transcription factors are prominent regulators of gene expression that execute responses to various intracellular and extracellular stimuli. Recombinant transcription reporter systems can be conveniently used to study the DNA binding preferences and regulatory activity of a transcription factor under a range of conditions. Several reporter genes have been used to study transcription regulation in the fission yeast Schizosaccharomyces pombe. Each of these reporters has distinct advantages, such as high sensitivity or ease of use, and limitations, such as prohibitive costs or use of hazardous substances. To combine the strengths and mitigate the weaknesses of individual reporter genes, we have created pREPORT, a flexible multi-readout transcription reporter vector for fission yeast that employs an enhanced GFP-lacZ fusion and a customizable minimal promoter. With pREPORT, gene expression driven by the transcription factor of interest can be quantified in a number of ways, both in live ce

Název v anglickém jazyce

pREPORT: a multi-readout transcription reporter vector for fission yeast

Popis výsledku anglicky

Transcription factors are prominent regulators of gene expression that execute responses to various intracellular and extracellular stimuli. Recombinant transcription reporter systems can be conveniently used to study the DNA binding preferences and regulatory activity of a transcription factor under a range of conditions. Several reporter genes have been used to study transcription regulation in the fission yeast Schizosaccharomyces pombe. Each of these reporters has distinct advantages, such as high sensitivity or ease of use, and limitations, such as prohibitive costs or use of hazardous substances. To combine the strengths and mitigate the weaknesses of individual reporter genes, we have created pREPORT, a flexible multi-readout transcription reporter vector for fission yeast that employs an enhanced GFP-lacZ fusion and a customizable minimal promoter. With pREPORT, gene expression driven by the transcription factor of interest can be quantified in a number of ways, both in live ce

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GPP305%2F12%2FP040" target="_blank" >GPP305/12/P040: Úloha proteinů CSL v udržování integrity genomu kvasinky Schizosaccharomyces pombe</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Yeast

ISSN

0749-503X

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

327-334

Kód UT WoS článku

000349022400002

EID výsledku v databázi Scopus

2-s2.0-84921712360