PKC alpha promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10315244" target="_blank" >RIV/00216208:11310/15:10315244 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s12885-015-1347-1" target="_blank" >http://dx.doi.org/10.1186/s12885-015-1347-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12885-015-1347-1" target="_blank" >10.1186/s12885-015-1347-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PKC alpha promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness

Popis výsledku v původním jazyce

Background: The local invasion of tumor cells into the surrounding tissue is the first and most critical step of the metastatic cascade. Cells can invade either collectively, or individually. Individual cancer cell invasion can occur in the mesenchymal or amoeboid mode, which are mutually interchangeable. This plasticity of individual cancer cell invasiveness may represent an escape mechanism for invading cancer cells from anti-metastatic treatment. Methods: To identify new signaling proteins involved in the plasticity of cancer cell invasiveness, we performed proteomic analysis of the amoeboid to mesenchymal transition with A375m2 melanoma cells in a 3D Matrigel matrix. Results: In this screen we identified PKC alpha as an important protein for the maintenance of amoeboid morphology. We found that the activation of PKCa resulted in the mesenchymal-amoeboid transition of mesenchymal K2 and MDA-MB-231 cell lines. Consistently, PKC alpha inhibition led to the amoeboid-mesenchymal transit

Název v anglickém jazyce

PKC alpha promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness

Popis výsledku anglicky

Background: The local invasion of tumor cells into the surrounding tissue is the first and most critical step of the metastatic cascade. Cells can invade either collectively, or individually. Individual cancer cell invasion can occur in the mesenchymal or amoeboid mode, which are mutually interchangeable. This plasticity of individual cancer cell invasiveness may represent an escape mechanism for invading cancer cells from anti-metastatic treatment. Methods: To identify new signaling proteins involved in the plasticity of cancer cell invasiveness, we performed proteomic analysis of the amoeboid to mesenchymal transition with A375m2 melanoma cells in a 3D Matrigel matrix. Results: In this screen we identified PKC alpha as an important protein for the maintenance of amoeboid morphology. We found that the activation of PKCa resulted in the mesenchymal-amoeboid transition of mesenchymal K2 and MDA-MB-231 cell lines. Consistently, PKC alpha inhibition led to the amoeboid-mesenchymal transit

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

—

Projekt

<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Cancer

ISSN

1471-2407

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

326

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000354016400001

EID výsledku v databázi Scopus

2-s2.0-84928790498