NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126706" target="_blank" >RIV/00216208:11310/12:10126706 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.ejcb.2012.05.001" target="_blank" >http://dx.doi.org/10.1016/j.ejcb.2012.05.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ejcb.2012.05.001" target="_blank" >10.1016/j.ejcb.2012.05.001</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells
Popis výsledku v původním jazyce
The aim of this study was to analyze the potential role of NG2 chondroitin sulfate proteoglycan in amoeboid morphology and invasiveness of cancer cells. In the highly metastatic arnoeboid cell lines A3 and A375M2, siRNA-mediated down-regulation of NG2 induced an amoeboid-mesenchymal transition associated with decreased invasiveness in 3D collagen and inactivation of the GTPase Rho. Conversely, the expression of NG2 in mesenchymal sarcoma K2 cells as well as in A375M2 cells resulted in an enhanced amoeboid phenotype associated with increased invasiveness and elevated Rho-GTP levels. Remarkably, the amoeboid-mesenchymal transition in A375M2 cells triggered by NG2 dovin-regulation was associated with increased extracellular matrix-degrading ability, although this was not sufficient to compensate for the decreased invasive capability caused by down-regulated Rho/ROCK signaling. Conversely, in K2 cells with overexpression of NG2, the ability to degrade the extracellular matrix was greatly r
Název v anglickém jazyce
NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells
Popis výsledku anglicky
The aim of this study was to analyze the potential role of NG2 chondroitin sulfate proteoglycan in amoeboid morphology and invasiveness of cancer cells. In the highly metastatic arnoeboid cell lines A3 and A375M2, siRNA-mediated down-regulation of NG2 induced an amoeboid-mesenchymal transition associated with decreased invasiveness in 3D collagen and inactivation of the GTPase Rho. Conversely, the expression of NG2 in mesenchymal sarcoma K2 cells as well as in A375M2 cells resulted in an enhanced amoeboid phenotype associated with increased invasiveness and elevated Rho-GTP levels. Remarkably, the amoeboid-mesenchymal transition in A375M2 cells triggered by NG2 dovin-regulation was associated with increased extracellular matrix-degrading ability, although this was not sufficient to compensate for the decreased invasive capability caused by down-regulated Rho/ROCK signaling. Conversely, in K2 cells with overexpression of NG2, the ability to degrade the extracellular matrix was greatly r
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EA - Morfologické obory a cytologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
European Journal of Cell Biology
ISSN
0171-9335
e-ISSN
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Svazek periodika
91
Číslo periodika v rámci svazku
11-12
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
969-977
Kód UT WoS článku
000311775000018
EID výsledku v databázi Scopus
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