Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10189096" target="_blank" >RIV/00216208:11310/13:10189096 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/13:00423218

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1478-811X-11-51" target="_blank" >http://dx.doi.org/10.1186/1478-811X-11-51</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1478-811X-11-51" target="_blank" >10.1186/1478-811X-11-51</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Popis výsledku v původním jazyce

Background: Although there is extensive evidence for the amoeboid invasiveness of cancer cells in vitro, much less is known about the role of amoeboid invasiveness in metastasis and the importance of Rho/ROCK/MLC signaling in this process. Results: We analyzed the dependence of amoeboid invasiveness of rat and chicken sarcoma cells and the metastatic activity of chicken cells on individual elements of the Rho/ROCK/MLC pathway. In both animal models, inhibition of Rho, ROCK or MLC resulted in greatly decreased cell invasiveness in vitro, while inhibition of extracellular proteases using a broad spectrum inhibitor did not have a significant effect. The inhibition of both Rho activity and MLC phosphorylation by dominant negative mutants led to a decreasedcapability of chicken sarcoma cells to metastasize. Moreover, the overexpression of RhoA in non-metastatic chicken cells resulted in the rescue of both invasiveness and metastatic capability. Rho and ROCK, unlike MLC, appeared to be dire

Název v anglickém jazyce

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Popis výsledku anglicky

Background: Although there is extensive evidence for the amoeboid invasiveness of cancer cells in vitro, much less is known about the role of amoeboid invasiveness in metastasis and the importance of Rho/ROCK/MLC signaling in this process. Results: We analyzed the dependence of amoeboid invasiveness of rat and chicken sarcoma cells and the metastatic activity of chicken cells on individual elements of the Rho/ROCK/MLC pathway. In both animal models, inhibition of Rho, ROCK or MLC resulted in greatly decreased cell invasiveness in vitro, while inhibition of extracellular proteases using a broad spectrum inhibitor did not have a significant effect. The inhibition of both Rho activity and MLC phosphorylation by dominant negative mutants led to a decreasedcapability of chicken sarcoma cells to metastasize. Moreover, the overexpression of RhoA in non-metastatic chicken cells resulted in the rescue of both invasiveness and metastatic capability. Rho and ROCK, unlike MLC, appeared to be dire

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06061" target="_blank" >LC06061: Centrum buněčné invazivity v embryonálním vývoji a metastázách nádorů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Communication and Signaling

ISSN

1478-811X

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

July 2013

Stát vydavatele periodika

IL - Stát Izrael

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

000322740600001

EID výsledku v databázi Scopus

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