Broad-range TRP channel inhibitors (2-APB, flufenamic acid, SKF-96365) affect differently contraction of resistance and conduit femoral arteries of rat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10316363" target="_blank" >RIV/00216208:11310/15:10316363 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/15:00451871

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ejphar.2015.09.014" target="_blank" >http://dx.doi.org/10.1016/j.ejphar.2015.09.014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejphar.2015.09.014" target="_blank" >10.1016/j.ejphar.2015.09.014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Broad-range TRP channel inhibitors (2-APB, flufenamic acid, SKF-96365) affect differently contraction of resistance and conduit femoral arteries of rat

Popis výsledku v původním jazyce

Transient receptor potential (TRP) channels are proposed to contribute to membrane depolarization and Ca2+ influx into vascular smooth muscle (VSM) cells. Our aim was to study the effects of widely used broad-range TRP channel inhibitors - 2-aminoethoxydiphenyl borate (2-APB), flufenamic acid (FFA) and SKF-96365 - on the contraction of freshly isolated small and large arteries. Endothelium-denuded resistance (approximate to 250 mu m) and conduit (approximate to 1000 mu m) femoral arteries were isolatedfrom adult Wistar rats and mounted in wire myograph. The effects of the above mentioned TRP channel inhibitors and voltage-dependent calcium channel inhibitor nifedipine were studied on arterial contractions induced by phenylephrine, U-46619 or K+. Phenylephrine-induced contractions were also studied in the absence of extracellular Na+ mRNA expression of particular canonical and melastatin TRP channel subunits in femoral vascular bed was determined. TRP channel inhibitors attenuated K+-i

Název v anglickém jazyce

Broad-range TRP channel inhibitors (2-APB, flufenamic acid, SKF-96365) affect differently contraction of resistance and conduit femoral arteries of rat

Popis výsledku anglicky

Transient receptor potential (TRP) channels are proposed to contribute to membrane depolarization and Ca2+ influx into vascular smooth muscle (VSM) cells. Our aim was to study the effects of widely used broad-range TRP channel inhibitors - 2-aminoethoxydiphenyl borate (2-APB), flufenamic acid (FFA) and SKF-96365 - on the contraction of freshly isolated small and large arteries. Endothelium-denuded resistance (approximate to 250 mu m) and conduit (approximate to 1000 mu m) femoral arteries were isolatedfrom adult Wistar rats and mounted in wire myograph. The effects of the above mentioned TRP channel inhibitors and voltage-dependent calcium channel inhibitor nifedipine were studied on arterial contractions induced by phenylephrine, U-46619 or K+. Phenylephrine-induced contractions were also studied in the absence of extracellular Na+ mRNA expression of particular canonical and melastatin TRP channel subunits in femoral vascular bed was determined. TRP channel inhibitors attenuated K+-i

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FA - Kardiovaskulární nemoci včetně kardiochirurgie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP304%2F12%2F0259" target="_blank" >GAP304/12/0259: Vzájemné působení jednotlivých vasoaktivních systémů, kyslíkových radikálů a G proteinů v experimentální hypertenzi</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Pharmacology

ISSN

0014-2999

e-ISSN

—

Svazek periodika

765

Číslo periodika v rámci svazku

Oct 15 (2015)

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

533-540

Kód UT WoS článku

000364249100064

EID výsledku v databázi Scopus

2-s2.0-84954357407