Simple electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10328322" target="_blank" >RIV/00216208:11310/15:10328322 - isvavai.cz</a>

Výsledek na webu

<a href="https://web.natur.cuni.cz/analchem/isc-mac/" target="_blank" >https://web.natur.cuni.cz/analchem/isc-mac/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simple electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals

Popis výsledku v původním jazyce

A novel simple electrochemical DNA biosensor based on a glassy carbon electrode was prepared by adsorbing low molecular weight double-stranded DNA from salmon sperm onto the electrode surface as a biorecognition layer. The biosensor was used for detection of DNA damage by hydroxyl radicals. Hydroxyl radicals are well-known reactive oxygen species inducing the oxidative stress in DNA. They can attack all the molecules, including DNA, and play a major role in the DNA oxidative damage. They were generated for our purposes electrochemically on the surface of a boron-doped diamond film electrode. A complex characterization of the specific damaging event was then obtained using a combination of several electrochemical detection techniques: cyclic voltammetry, square-wave voltammetry, and electrochemical impedance spectroscopy.

Název v anglickém jazyce

Simple electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals

Popis výsledku anglicky

A novel simple electrochemical DNA biosensor based on a glassy carbon electrode was prepared by adsorbing low molecular weight double-stranded DNA from salmon sperm onto the electrode surface as a biorecognition layer. The biosensor was used for detection of DNA damage by hydroxyl radicals. Hydroxyl radicals are well-known reactive oxygen species inducing the oxidative stress in DNA. They can attack all the molecules, including DNA, and play a major role in the DNA oxidative damage. They were generated for our purposes electrochemically on the surface of a boron-doped diamond film electrode. A complex characterization of the specific damaging event was then obtained using a combination of several electrochemical detection techniques: cyclic voltammetry, square-wave voltammetry, and electrochemical impedance spectroscopy.

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Centrum nových přístupů k bioanalýze a molekulární diagnostice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

PROCEEDINGS OF THE 11TH INTERNATIONAL STUDENTS CONFERENCE "MODERN ANALYTICAL CHEMISTRY"

ISBN

978-80-7444-036-6

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

19-22

Název nakladatele

CHARLES UNIV, FAC SCIENCE

Místo vydání

PRAGUE 2

Místo konání akce

Prague

Datum konání akce

22. 9. 2015

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000383853900004