Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10297240" target="_blank" >RIV/00216208:11310/16:10297240 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-015-1590-9" target="_blank" >http://dx.doi.org/10.1007/s00706-015-1590-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-015-1590-9" target="_blank" >10.1007/s00706-015-1590-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Popis výsledku v původním jazyce

Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm(-3) acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV-Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction ofMV and the DNA-MV complex and the thermodynamic parameters of the formed DNA-MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution. [GRAPHICS] .

Název v anglickém jazyce

Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Popis výsledku anglicky

Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm(-3) acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV-Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction ofMV and the DNA-MV complex and the thermodynamic parameters of the formed DNA-MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution. [GRAPHICS] .

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Centrum nových přístupů k bioanalýze a molekulární diagnostice</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

119-126

Kód UT WoS článku

000367521400016

EID výsledku v databázi Scopus

2-s2.0-84952802369