Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10371535" target="_blank" >RIV/00216208:11310/16:10371535 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/16:00461980 RIV/60076658:12310/16:43890612

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.nano.2015.09.008" target="_blank" >http://dx.doi.org/10.1016/j.nano.2015.09.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nano.2015.09.008" target="_blank" >10.1016/j.nano.2015.09.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

Popis výsledku v původním jazyce

We present a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals. From the Clinical Editor: The use of transmission electron microscopy has become an indispensible tool in the detection of cellular proteins. In this short but interesting article, the authors described their new method of labeling and the identification of four different proteins simultaneously, which represents another advance in imaging technique.

Název v anglickém jazyce

Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

Popis výsledku anglicky

We present a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals. From the Clinical Editor: The use of transmission electron microscopy has become an indispensible tool in the detection of cellular proteins. In this short but interesting article, the authors described their new method of labeling and the identification of four different proteins simultaneously, which represents another advance in imaging technique.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanomedicine: Nanotechnology, Biology, and Medicine

ISSN

1549-9634

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

105-108

Kód UT WoS článku

000371800600010

EID výsledku v databázi Scopus

2-s2.0-84957064578