Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F15%3A00449551" target="_blank" >RIV/68081731:_____/15:00449551 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s13594-015-0253-2" target="_blank" >http://dx.doi.org/10.1007/s13594-015-0253-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13594-015-0253-2" target="_blank" >10.1007/s13594-015-0253-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Popis výsledku v původním jazyce

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allowfor precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

Název v anglickém jazyce

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Popis výsledku anglicky

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allowfor precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Dairy Science & Technology

ISSN

1958-5586

e-ISSN

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Svazek periodika

95

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

14

Strana od-do

687-700

Kód UT WoS článku

000361480900009

EID výsledku v databázi Scopus

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