Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10368252" target="_blank" >RIV/00216208:11310/17:10368252 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1021/acs.analchem.6b04172" target="_blank" >https://doi.org/10.1021/acs.analchem.6b04172</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.analchem.6b04172" target="_blank" >10.1021/acs.analchem.6b04172</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification

Popis výsledku v původním jazyce

To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BGE) instead of the sample thus eliminating the need for additional sample manipulation, microchip redesigns, and/or system expansions required for traditional ISTD usage. Cs and Li ions were added as integrated ISTDs to the BGE, and their effects on the reproducibility of Na quantification were explored. Results were then compared to the conclusions of our previous publication which used Cs and Li as traditional ISTDs. The inhouse fabricated microchips, electrophoretic protocols, and solution matrixes were kept constant, allowing the proposed method to be reliably compared to the traditional method. Using the integrated ISTDs, both Cs and Li improved the Na peak area reproducibility approximately 2 -fold, to final RSD values of 2.2-4.7% (n = 900). In contrast (to previous work), Cs as a traditional ISTD resulted in final RSDs of 2.5-8.8%, while the traditional Li ISTD performed poorly with RSDs of 6.3-14.2%. These findings suggest integrated ISTDs are a viable method to improve the precision of disposable MCE devices giving matched or superior results to the traditional method in this study while neither increasing system cost nor complexity.

Název v anglickém jazyce

Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification

Popis výsledku anglicky

To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BGE) instead of the sample thus eliminating the need for additional sample manipulation, microchip redesigns, and/or system expansions required for traditional ISTD usage. Cs and Li ions were added as integrated ISTDs to the BGE, and their effects on the reproducibility of Na quantification were explored. Results were then compared to the conclusions of our previous publication which used Cs and Li as traditional ISTDs. The inhouse fabricated microchips, electrophoretic protocols, and solution matrixes were kept constant, allowing the proposed method to be reliably compared to the traditional method. Using the integrated ISTDs, both Cs and Li improved the Na peak area reproducibility approximately 2 -fold, to final RSD values of 2.2-4.7% (n = 900). In contrast (to previous work), Cs as a traditional ISTD resulted in final RSDs of 2.5-8.8%, while the traditional Li ISTD performed poorly with RSDs of 6.3-14.2%. These findings suggest integrated ISTDs are a viable method to improve the precision of disposable MCE devices giving matched or superior results to the traditional method in this study while neither increasing system cost nor complexity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GJ15-18424Y" target="_blank" >GJ15-18424Y: Vývoj nové generace software a pokročilé teorie elektromigrace v metodách elektrokinetické chromatografie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

2886-2892

Kód UT WoS článku

000395964400033

EID výsledku v databázi Scopus

2-s2.0-85026778736