An integrated microfluidic chip for immunocapture, preconcentration and separation of ?-amyloid peptides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900560" target="_blank" >RIV/00216275:25310/15:39900560 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1063/1.4931394" target="_blank" >http://dx.doi.org/10.1063/1.4931394</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/1.4931394" target="_blank" >10.1063/1.4931394</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An integrated microfluidic chip for immunocapture, preconcentration and separation of ?-amyloid peptides

Popis výsledku v původním jazyce

We present an integrated microfluidic chip for detection of ?-amyloid (A?) peptides. A? peptides are major biomarkers for the diagnosis of Alzheimer's disease (AD) in its early stages. This microfluidic device consists of three main parts: (1) An immunocapture microcolumn based on self-assembled magnetic beads coated with antibodies specific to A? peptides, (2) a nano-porous membrane made of photopolymerized hydrogel for preconcentration, and (3) a microchip electrophoresis (MCE) channel with fluorescent detection. Sub-milliliter sample volume is either mixed off-chip with antibody coated magnetic beads and injected into the device or is injected into an already self-assembled column of magnetic beads in the microchannel. The captured peptides on the beads are then electrokinetically eluted and re-concentrated onto the nano-membrane in a few nano-liters. By integrating the nano-membrane, total assay time was reduced and also off-chip re-concentration or buffer exchange steps were not n

Název v anglickém jazyce

An integrated microfluidic chip for immunocapture, preconcentration and separation of ?-amyloid peptides

Popis výsledku anglicky

We present an integrated microfluidic chip for detection of ?-amyloid (A?) peptides. A? peptides are major biomarkers for the diagnosis of Alzheimer's disease (AD) in its early stages. This microfluidic device consists of three main parts: (1) An immunocapture microcolumn based on self-assembled magnetic beads coated with antibodies specific to A? peptides, (2) a nano-porous membrane made of photopolymerized hydrogel for preconcentration, and (3) a microchip electrophoresis (MCE) channel with fluorescent detection. Sub-milliliter sample volume is either mixed off-chip with antibody coated magnetic beads and injected into the device or is injected into an already self-assembled column of magnetic beads in the microchannel. The captured peptides on the beads are then electrokinetically eluted and re-concentrated onto the nano-membrane in a few nano-liters. By integrating the nano-membrane, total assay time was reduced and also off-chip re-concentration or buffer exchange steps were not n

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/7E12083" target="_blank" >7E12083: NAnosystems for the early DIagnosis of NEurodegenerative Diseases</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomicrofluidics

ISSN

1932-1058

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

054117

Kód UT WoS článku

000364407300029

EID výsledku v databázi Scopus

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