Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39894651" target="_blank" >RIV/00216275:25310/12:39894651 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1063/1.4722588" target="_blank" >http://dx.doi.org/10.1063/1.4722588</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/1.4722588" target="_blank" >10.1063/1.4722588</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers

Popis výsledku v původním jazyce

Determination of amyloid beta (Abeta) isoforms - and in particular the proportion of the Abeta 1-42 isoform - in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease (AD) might help in early diagnosis and treatment of that illness. Dueto the low concentration of Abeta peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (mýIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Abeta antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the properbeads and anti-Abeta antibodies, as well as optimizing the immobilization technique and mýIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Abeta IS for mýIP and prec

Název v anglickém jazyce

Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers

Popis výsledku anglicky

Determination of amyloid beta (Abeta) isoforms - and in particular the proportion of the Abeta 1-42 isoform - in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease (AD) might help in early diagnosis and treatment of that illness. Dueto the low concentration of Abeta peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (mýIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Abeta antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the properbeads and anti-Abeta antibodies, as well as optimizing the immobilization technique and mýIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Abeta IS for mýIP and prec

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomicrofluidics

ISSN

1932-1058

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

"024126-1"-"024126-12"

Kód UT WoS článku

000305839800037

EID výsledku v databázi Scopus

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