FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10388456" target="_blank" >RIV/00216208:11310/18:10388456 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1016/j.bpj.2018.04.050" target="_blank" >https://doi.org/10.1016/j.bpj.2018.04.050</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2018.04.050" target="_blank" >10.1016/j.bpj.2018.04.050</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells
Popis výsledku v původním jazyce
The coexistence of lipid domains with different degrees of lipid packing in the plasma membrane of mammalian cells has been postulated, but direct evidence has so far been challenging to obtain because of the small size and short lifetime of these domains in live cells. Here, we use fluorescence spectral correlation spectroscopy in conjunction with a probe sensitive to the membrane environment to quantify spectral fluctuations associated with dynamics of membrane domains in live cells. With this method, we show that membrane domains are present in live COS-7 cells and have a lifetime lower bound of 5.90 and 14.69 ms for the ordered and disordered phases, respectively. Comparisons to simulations indicate that the underlying mechanism of these fluctuations is complex but qualitatively described by a combination of dye diffusion between membrane domains as well as the motion of domains within the membrane.
Název v anglickém jazyce
FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells
Popis výsledku anglicky
The coexistence of lipid domains with different degrees of lipid packing in the plasma membrane of mammalian cells has been postulated, but direct evidence has so far been challenging to obtain because of the small size and short lifetime of these domains in live cells. Here, we use fluorescence spectral correlation spectroscopy in conjunction with a probe sensitive to the membrane environment to quantify spectral fluctuations associated with dynamics of membrane domains in live cells. With this method, we show that membrane domains are present in live COS-7 cells and have a lifetime lower bound of 5.90 and 14.69 ms for the ordered and disordered phases, respectively. Comparisons to simulations indicate that the underlying mechanism of these fluctuations is complex but qualitatively described by a combination of dye diffusion between membrane domains as well as the motion of domains within the membrane.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
—
Návaznosti
O - Projekt operacniho programu
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biophysical Journal
ISSN
0006-3495
e-ISSN
—
Svazek periodika
114
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
2855-2864
Kód UT WoS článku
000436471800012
EID výsledku v databázi Scopus
2-s2.0-85048497774