Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F17%3A00476382" target="_blank" >RIV/61388955:_____/17:00476382 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s41598-017-05539-y" target="_blank" >http://dx.doi.org/10.1038/s41598-017-05539-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-05539-y" target="_blank" >10.1038/s41598-017-05539-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered

Popis výsledku v původním jazyce

It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesteroln(Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on modelnmembranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although thenexistence of such microdomains has not been proven for the plasma membrane, such lipid mixturesnhave been often used as a model system for ‘rafts’. On the other hand, recent super resolution andnsingle molecule results indicate that the plasma membrane might organize into nanocompartments.nHowever, due to the limited resolution of those techniques their unambiguous characterizationnis still missing. In this work, a novel combination of Förster resonance energy transfer and MontenCarlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered modelnmembranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solidstatenwide-line and high resolution magic angle spinning NMR as well as with fluorescence correlationnspectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluidnand disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent anrelevant model system for cellular membranes and are closely related to nanocompartments suggestednto exist in cellular membranes.

Název v anglickém jazyce

Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered

Popis výsledku anglicky

It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesteroln(Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on modelnmembranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although thenexistence of such microdomains has not been proven for the plasma membrane, such lipid mixturesnhave been often used as a model system for ‘rafts’. On the other hand, recent super resolution andnsingle molecule results indicate that the plasma membrane might organize into nanocompartments.nHowever, due to the limited resolution of those techniques their unambiguous characterizationnis still missing. In this work, a novel combination of Förster resonance energy transfer and MontenCarlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered modelnmembranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solidstatenwide-line and high resolution magic angle spinning NMR as well as with fluorescence correlationnspectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluidnand disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent anrelevant model system for cellular membranes and are closely related to nanocompartments suggestednto exist in cellular membranes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GA17-03160S" target="_blank" >GA17-03160S: Role gangliosidů ve vývoji neurodegenerativních amyloidogenních nemocí: molekulární pohled pomocí pokročilé fluorescenční mikroskopie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

JUL 2017

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

000405464200097

EID výsledku v databázi Scopus

2-s2.0-85024403161