Heterogeneous Lipid Distributions in Membranes as Revealed by Electronic Energy Transfer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F16%3A00457553" target="_blank" >RIV/61388955:_____/16:00457553 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-3-319-24609-3_7" target="_blank" >http://dx.doi.org/10.1007/978-3-319-24609-3_7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-319-24609-3_7" target="_blank" >10.1007/978-3-319-24609-3_7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Heterogeneous Lipid Distributions in Membranes as Revealed by Electronic Energy Transfer

Popis výsledku v původním jazyce

The techniques achieving the highest resolution only can characterize/nmembrane heterogeneities on the lowest molecular level. When F€orster resonance/nenergy transfer (FRET) is combined with Monte-Carlo (MC) simulations and is/napplied to the measurement of nanodomain/pore sizes it reaches an unbeatable/nresolution of 2–50 nm. While other techniques start being less efficient at such/nshort distances FRET is most efficient in this region. Here, usefulness of/nMC-FRET is demonstrated on three different systems that contain heterogeneously/ndistributed lipids: a nanoscopically phase separated bilayer, a bilayer containing/npores and finally on bicelles consisting of highly curved and flat regions. Moreover,/nthis paper gives the reader information on how a FRET experiment should be/ndesigned to achieve the highest FRET resolution but also which experimental/nconditions should be avoided. The theory describing FRET between randomly/ndistributed donors and acceptors in a lipid bilayer is also described in this paper as/nwell as reasons are explained why for heterogeneous probe distribution MC simulations/nshould rather be used.

Název v anglickém jazyce

Heterogeneous Lipid Distributions in Membranes as Revealed by Electronic Energy Transfer

Popis výsledku anglicky

The techniques achieving the highest resolution only can characterize/nmembrane heterogeneities on the lowest molecular level. When F€orster resonance/nenergy transfer (FRET) is combined with Monte-Carlo (MC) simulations and is/napplied to the measurement of nanodomain/pore sizes it reaches an unbeatable/nresolution of 2–50 nm. While other techniques start being less efficient at such/nshort distances FRET is most efficient in this region. Here, usefulness of/nMC-FRET is demonstrated on three different systems that contain heterogeneously/ndistributed lipids: a nanoscopically phase separated bilayer, a bilayer containing/npores and finally on bicelles consisting of highly curved and flat regions. Moreover,/nthis paper gives the reader information on how a FRET experiment should be/ndesigned to achieve the highest FRET resolution but also which experimental/nconditions should be avoided. The theory describing FRET between randomly/ndistributed donors and acceptors in a lipid bilayer is also described in this paper as/nwell as reasons are explained why for heterogeneous probe distribution MC simulations/nshould rather be used.

Druh

C - Kapitola v odborné knize

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GC14-03141J" target="_blank" >GC14-03141J: Studium vztahu struktury a funkce FGF2 oligomerů tvořících membránové póry technikami zaměřenými na sledování jedné molekuly</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Reviews in Fluorescence 2015

ISBN

978-3-319-24609-3

Počet stran výsledku

17

Strana od-do

171-187

Počet stran knihy

367

Název nakladatele

Springer

Místo vydání

New York

Kód UT WoS kapitoly

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