The Analysis of In-Membrane Nanoscopic Aggregation of Lipids and Proteins by MC-FRET

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00571347" target="_blank" >RIV/61388955:_____/23:00571347 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/23:00571347 RIV/00216208:11320/22:10447432

Výsledek na webu

<a href="http://dx.doi.org/10.1007/4243_2022_29" target="_blank" >http://dx.doi.org/10.1007/4243_2022_29</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/4243_2022_29" target="_blank" >10.1007/4243_2022_29</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Analysis of In-Membrane Nanoscopic Aggregation of Lipids and Proteins by MC-FRET

Popis výsledku v původním jazyce

Many lipids and membrane proteins spontaneously co-cluster and oligomerize in cellular plasma membranes into larger (functional) units, whose detailed characterization requires high spatial resolution. In this contribution, we introduce a powerful spectroscopy/microscopy approach called MC-FRET developed for the analysis of in-membrane nanoscopic aggregation of lipids and proteins in biological membranes. The approach is based on Förster resonance energy transfer (FRET) occurring in membranes between fluorescently labelled lipids/proteins and subsequent analysis of data by Monte-Carlo simulations. The following applications of MC-FRET are presented here: (1) determination of lipid nanodomain sizes and their surface density, (2) characterization of inter-leaflet organization of lipid nanodomains and (3) the analysis of dimerization of in-membrane proteins.

Název v anglickém jazyce

The Analysis of In-Membrane Nanoscopic Aggregation of Lipids and Proteins by MC-FRET

Popis výsledku anglicky

Many lipids and membrane proteins spontaneously co-cluster and oligomerize in cellular plasma membranes into larger (functional) units, whose detailed characterization requires high spatial resolution. In this contribution, we introduce a powerful spectroscopy/microscopy approach called MC-FRET developed for the analysis of in-membrane nanoscopic aggregation of lipids and proteins in biological membranes. The approach is based on Förster resonance energy transfer (FRET) occurring in membranes between fluorescently labelled lipids/proteins and subsequent analysis of data by Monte-Carlo simulations. The following applications of MC-FRET are presented here: (1) determination of lipid nanodomain sizes and their surface density, (2) characterization of inter-leaflet organization of lipid nanodomains and (3) the analysis of dimerization of in-membrane proteins.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Fluorescence spectroscopy and microscopy in biology

ISBN

978-3-031-30361-6

Počet stran výsledku

26

Strana od-do

375-400

Počet stran knihy

532

Název nakladatele

Springer Nature

Místo vydání

Chum

Kód UT WoS kapitoly

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