Mapping of Hieracium (Asteraceae) chromosomes with genus-specific satDNA elements derived from next-generation sequencing data

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10388478" target="_blank" >RIV/00216208:11310/18:10388478 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/18:00492191

Výsledek na webu

<a href="https://doi.org/10.1007/s00606-017-1483-y" target="_blank" >https://doi.org/10.1007/s00606-017-1483-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00606-017-1483-y" target="_blank" >10.1007/s00606-017-1483-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mapping of Hieracium (Asteraceae) chromosomes with genus-specific satDNA elements derived from next-generation sequencing data

Popis výsledku v původním jazyce

The highly repetitive DNA fraction of the eukaryotic genome is considered a mobile, rapidly changing entity, thus reflecting trajectories of short-term evolutionary change. It consists of several large classes in which transposable elements and satellite DNA (satDNA) predominate. Despite a growing awareness of its structure and functional significance, the evolutionary dynamics of repetitive elements and, particularly, satDNA remain poorly characterized. Next-generation sequencing (NGS) has opened up new possibilities for high-throughput genome analysis. Here, we applied satDNA repeatome elements derived from NGS data as probes for fluorescence in situ hybridization to characterize the karyotypes of three diploid hawkweed species of the predominantly polyploid apomictic genus Hieracium, namely H. intybaceum, H. prenanthoides and H. alpinum. Three cluster-distributed, genus-specific satDNA elements that are not present in the sister genus Pilosella were identified; notably, one element spans the functional centromeres. Each of the investigated diploids possessed a species-specific assortment of detected repeats. Their utilization as molecular-cytogenetic markers, in combination with ribosomal DNA loci, allowed for the development of a system to identify the individual chromosomes of the Hieracium species, thus providing a basis for the future investigation of karyotype evolution in diploid hawkweeds and for exploring satDNA dynamics in hybrids and apomicts of allopolyploid origin.

Název v anglickém jazyce

Mapping of Hieracium (Asteraceae) chromosomes with genus-specific satDNA elements derived from next-generation sequencing data

Popis výsledku anglicky

The highly repetitive DNA fraction of the eukaryotic genome is considered a mobile, rapidly changing entity, thus reflecting trajectories of short-term evolutionary change. It consists of several large classes in which transposable elements and satellite DNA (satDNA) predominate. Despite a growing awareness of its structure and functional significance, the evolutionary dynamics of repetitive elements and, particularly, satDNA remain poorly characterized. Next-generation sequencing (NGS) has opened up new possibilities for high-throughput genome analysis. Here, we applied satDNA repeatome elements derived from NGS data as probes for fluorescence in situ hybridization to characterize the karyotypes of three diploid hawkweed species of the predominantly polyploid apomictic genus Hieracium, namely H. intybaceum, H. prenanthoides and H. alpinum. Three cluster-distributed, genus-specific satDNA elements that are not present in the sister genus Pilosella were identified; notably, one element spans the functional centromeres. Each of the investigated diploids possessed a species-specific assortment of detected repeats. Their utilization as molecular-cytogenetic markers, in combination with ribosomal DNA loci, allowed for the development of a system to identify the individual chromosomes of the Hieracium species, thus providing a basis for the future investigation of karyotype evolution in diploid hawkweeds and for exploring satDNA dynamics in hybrids and apomicts of allopolyploid origin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Systematics and Evolution

ISSN

0378-2697

e-ISSN

—

Svazek periodika

304

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

10

Strana od-do

387-396

Kód UT WoS článku

000426073500007

EID výsledku v databázi Scopus

2-s2.0-85035318879