Characterization and dynamics of repeatomes in closely related species of Hieracium (Asteraceae) and their synthetic and apomictic hybrids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00533842" target="_blank" >RIV/67985939:_____/20:00533842 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3389/fpls.2020.591053" target="_blank" >https://doi.org/10.3389/fpls.2020.591053</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2020.591053" target="_blank" >10.3389/fpls.2020.591053</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization and dynamics of repeatomes in closely related species of Hieracium (Asteraceae) and their synthetic and apomictic hybrids

Popis výsledku v původním jazyce

Non-model plants with large genomes have not often been studied at the genomic level. Here we present the first characterization of repeatomes in hawkweeds (Hieracium), Asteraceae with highly repetitive genomes. We use a model system, which enables us to distinguish the effects of hybridization from those of polyploidization, the latter coupled with the apomictic mode of reproduction. We study the dynamics of repetitive elements in two parents and their diploid synthetic F1 and triploid apomictic natural hybrids using NGS data and cytogenetic analyses of satellite DNA. We found that the kind and abundance of transposable elements, the largest fraction of the genomes, were basically unaffected by both evolutionary processes (i.e., no bursts were observed), but pararetrovirus was overabundant in apomicts and may be involved in polyploidization and/or a switch to asexual reproduction. Ribosomal and satellite DNAs were under- or overrepresented in hybrid/allopolyploid genomes and did not always correspond to the presence and number of chromosomal loci. Most of these patterns were individual-specific, but some satellites appeared to be species-specific, highlighting the highly dynamic nature of satellite DNA. This work contributes to the general understanding of repeatome evolution in polyploid apomictic plants, which is so far understudied.

Název v anglickém jazyce

Characterization and dynamics of repeatomes in closely related species of Hieracium (Asteraceae) and their synthetic and apomictic hybrids

Popis výsledku anglicky

Non-model plants with large genomes have not often been studied at the genomic level. Here we present the first characterization of repeatomes in hawkweeds (Hieracium), Asteraceae with highly repetitive genomes. We use a model system, which enables us to distinguish the effects of hybridization from those of polyploidization, the latter coupled with the apomictic mode of reproduction. We study the dynamics of repetitive elements in two parents and their diploid synthetic F1 and triploid apomictic natural hybrids using NGS data and cytogenetic analyses of satellite DNA. We found that the kind and abundance of transposable elements, the largest fraction of the genomes, were basically unaffected by both evolutionary processes (i.e., no bursts were observed), but pararetrovirus was overabundant in apomicts and may be involved in polyploidization and/or a switch to asexual reproduction. Ribosomal and satellite DNAs were under- or overrepresented in hybrid/allopolyploid genomes and did not always correspond to the presence and number of chromosomal loci. Most of these patterns were individual-specific, but some satellites appeared to be species-specific, highlighting the highly dynamic nature of satellite DNA. This work contributes to the general understanding of repeatome evolution in polyploid apomictic plants, which is so far understudied.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/GA17-14620S" target="_blank" >GA17-14620S: Dynamika repetitivních sekvencí v raných stadiích evoluce apomiktických rostlin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

NOV 2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

21

Strana od-do

1-21

Kód UT WoS článku

000589348200001

EID výsledku v databázi Scopus

2-s2.0-85096052761