Batch Injection Analysis with Amperometric Detection for DNA Biosensing Applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10396636" target="_blank" >RIV/00216208:11310/19:10396636 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2a3U73d7U3" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2a3U73d7U3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201900279" target="_blank" >10.1002/elan.201900279</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Batch Injection Analysis with Amperometric Detection for DNA Biosensing Applications

Popis výsledku v původním jazyce

In this work, batch injection analysis with the amperometric detection (BIA-AD), employing a detection cell designed to adapt a screen-printed carbon electrode (SPCE) was used for the first time as a robust electroanalytical system for DNA biosensing applications. The sensitive amperometric detection was used to evaluate the structural changes in double-stranded DNA (dsDNA) after UV-C irradiation of its solution for a given time. Batching of DNA samples was performed by precise electronic pipette microinjection of an irradiated sample aliquot onto the unmodified activated SPCE surface incorporated in the BIA-AD system. Using the optimized experimental conditions (40 mu L of 1 mg mL(-1) dsDNA in a 0.1 M phosphate buffer of pH 7.4 sampled at the injection speed degree of 6 and detected at the potential of +1.5 V vs silver pseudo-reference electrode), a time-dependent response (gradual decrease of amperometric signal up to 58 % after 10 min of the irradiation) was found for the detection of damage to low molecular weight salmon sperm dsDNA. The advantages of this low-dimensional and cost-effective measuring system can be utilized not only for the quantification of DNA damage/degradation by UV irradiation, but they are also promising for studying other types of DNA interactions.

Název v anglickém jazyce

Batch Injection Analysis with Amperometric Detection for DNA Biosensing Applications

Popis výsledku anglicky

In this work, batch injection analysis with the amperometric detection (BIA-AD), employing a detection cell designed to adapt a screen-printed carbon electrode (SPCE) was used for the first time as a robust electroanalytical system for DNA biosensing applications. The sensitive amperometric detection was used to evaluate the structural changes in double-stranded DNA (dsDNA) after UV-C irradiation of its solution for a given time. Batching of DNA samples was performed by precise electronic pipette microinjection of an irradiated sample aliquot onto the unmodified activated SPCE surface incorporated in the BIA-AD system. Using the optimized experimental conditions (40 mu L of 1 mg mL(-1) dsDNA in a 0.1 M phosphate buffer of pH 7.4 sampled at the injection speed degree of 6 and detected at the potential of +1.5 V vs silver pseudo-reference electrode), a time-dependent response (gradual decrease of amperometric signal up to 58 % after 10 min of the irradiation) was found for the detection of damage to low molecular weight salmon sperm dsDNA. The advantages of this low-dimensional and cost-effective measuring system can be utilized not only for the quantification of DNA damage/degradation by UV irradiation, but they are also promising for studying other types of DNA interactions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Svazek periodika

31

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

2001-2006

Kód UT WoS článku

000476014600001

EID výsledku v databázi Scopus

2-s2.0-85069713548