Determination of low-molecular-mass heparin using affinity capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409584" target="_blank" >RIV/00216208:11310/19:10409584 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc15.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc15.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of low-molecular-mass heparin using affinity capillary electrophoresis

Popis výsledku v původním jazyce

This work is dedicated to determination of low-molecular-mass heparin, namely Fraxiparine, using affinity capillary electrophoresis. Fraxiparine was detected indirectly by using tetraarginine, which forms a stable complex with Fraxiparine. The developed method used 50 μm i.d. fused silica capillary (length was optimized). Phosphoric acid (9 mM) with addition of 0.1% w/v hydroxyethylcellulose was used as a background electrolyte. The samples were injected hydrodynamically into the capillary by a pressure of 5 kPa. After that, voltage was applied for a certain period of time. During this time the zones migrate through each other, which enables formation of the complex. Fraxiparine was determined from the amount of remaining free tetraarginine. The injection time of Fraxiparine was subject to optimization. After the optimization, different experiments were performed for the determination of Fraxiparine in blood plasma. Acetonitrile, acetone and trifluoroacetic acid were tested for deproteination of the samples.

Název v anglickém jazyce

Determination of low-molecular-mass heparin using affinity capillary electrophoresis

Popis výsledku anglicky

This work is dedicated to determination of low-molecular-mass heparin, namely Fraxiparine, using affinity capillary electrophoresis. Fraxiparine was detected indirectly by using tetraarginine, which forms a stable complex with Fraxiparine. The developed method used 50 μm i.d. fused silica capillary (length was optimized). Phosphoric acid (9 mM) with addition of 0.1% w/v hydroxyethylcellulose was used as a background electrolyte. The samples were injected hydrodynamically into the capillary by a pressure of 5 kPa. After that, voltage was applied for a certain period of time. During this time the zones migrate through each other, which enables formation of the complex. Fraxiparine was determined from the amount of remaining free tetraarginine. The injection time of Fraxiparine was subject to optimization. After the optimization, different experiments were performed for the determination of Fraxiparine in blood plasma. Acetonitrile, acetone and trifluoroacetic acid were tested for deproteination of the samples.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 15th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-068-7

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

39-44

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

19. 9. 2019

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000576787700006