Interaction of heparin and tetraarginine in capillary electrophoresis: Implication for analytical applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10419218" target="_blank" >RIV/00216208:11310/20:10419218 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YIu1DWJqD0" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YIu1DWJqD0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.202000011" target="_blank" >10.1002/elps.202000011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of heparin and tetraarginine in capillary electrophoresis: Implication for analytical applications

Popis výsledku v původním jazyce

Interactions between heparin and tetraarginine in an acidic background electrolyte were investigated in capillary electrophoresis. The results showed that tetraarginine and heparin form a stable complex that migrates toward the anode immediately after coming into contact. When a zone of tetraarginine at a mg/mL concentration level passes through a zone of heparin at a mu g/mL concentration level, tetraarginine is gradually removed by the formation of the complex that migrates in the opposite direction, thereby decreasing the tetraarginine peak area. The variation of the tetraarginine peak area as a function of the unfractionated heparin concentration was linear within the range 2-20 mu g/mL, which enables us to detect and determine heparin concentrations undetectable with a UV detector. The same behavior was confirmed for low molecular weight heparin.

Název v anglickém jazyce

Interaction of heparin and tetraarginine in capillary electrophoresis: Implication for analytical applications

Popis výsledku anglicky

Interactions between heparin and tetraarginine in an acidic background electrolyte were investigated in capillary electrophoresis. The results showed that tetraarginine and heparin form a stable complex that migrates toward the anode immediately after coming into contact. When a zone of tetraarginine at a mg/mL concentration level passes through a zone of heparin at a mu g/mL concentration level, tetraarginine is gradually removed by the formation of the complex that migrates in the opposite direction, thereby decreasing the tetraarginine peak area. The variation of the tetraarginine peak area as a function of the unfractionated heparin concentration was linear within the range 2-20 mu g/mL, which enables us to detect and determine heparin concentrations undetectable with a UV detector. The same behavior was confirmed for low molecular weight heparin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

21-22

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

1826-1831

Kód UT WoS článku

000556918100001

EID výsledku v databázi Scopus

2-s2.0-85083842198