Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10410695" target="_blank" >RIV/00216208:11310/20:10410695 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11110/20:10410695 RIV/00023728:_____/20:N0000058
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=fFS_Aw8XCr" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=fFS_Aw8XCr</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ab.2019.113509" target="_blank" >10.1016/j.ab.2019.113509</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress
Popis výsledku v původním jazyce
Allantoin is an excellent biomarker of oxidative stress in humans as the main product of uric acid oxidation by reactive oxygen species. Yet, allantoin determination is still not routinely performed in clinical laboratories. Therefore, we developed a fast, simple, selective, and sensitive UHPLC-MS/MS method for allantoin determination in human serum using an isotopically labeled internal standard. Our analytical protocol provided high sensitivity by mass spectrometry detection and high throughput by HILIC-MS/MS analysis within 4 min, with one-step serum sample preparation approximately within 7 min. Lastly, our protocol was fully validated to demonstrate its reliability in allantoin determination in human serum. The method showed an excellent linear range from 0.05 to 100 mu M, with precision ranging from 1.8 to 11.3% (RSD), and with accuracy (relative error %) within +/- 6.0%. The method was then applied to analyze the concentration of allantoin in serum samples from 71 patients with chronic gout without treatment with xanthine oxidase inhibitors. The median serum allantoin concentration in the cohort was 2.8 mu M (n = 71). Overall, our simple analytical protocol has the potential to be easily implemented in clinical routine practice for monitoring allantoin as a key oxidative stress biomarker.
Název v anglickém jazyce
Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress
Popis výsledku anglicky
Allantoin is an excellent biomarker of oxidative stress in humans as the main product of uric acid oxidation by reactive oxygen species. Yet, allantoin determination is still not routinely performed in clinical laboratories. Therefore, we developed a fast, simple, selective, and sensitive UHPLC-MS/MS method for allantoin determination in human serum using an isotopically labeled internal standard. Our analytical protocol provided high sensitivity by mass spectrometry detection and high throughput by HILIC-MS/MS analysis within 4 min, with one-step serum sample preparation approximately within 7 min. Lastly, our protocol was fully validated to demonstrate its reliability in allantoin determination in human serum. The method showed an excellent linear range from 0.05 to 100 mu M, with precision ranging from 1.8 to 11.3% (RSD), and with accuracy (relative error %) within +/- 6.0%. The method was then applied to analyze the concentration of allantoin in serum samples from 71 patients with chronic gout without treatment with xanthine oxidase inhibitors. The median serum allantoin concentration in the cohort was 2.8 mu M (n = 71). Overall, our simple analytical protocol has the potential to be easily implemented in clinical routine practice for monitoring allantoin as a key oxidative stress biomarker.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GJ19-18005Y" target="_blank" >GJ19-18005Y: Retenční mechanismus intaktních glykopeptidů v hydrofilní interakční kapalinové chromatografii</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytical Biochemistry
ISSN
0003-2697
e-ISSN
—
Svazek periodika
589
Číslo periodika v rámci svazku
January
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
5
Strana od-do
113509
Kód UT WoS článku
000518179100012
EID výsledku v databázi Scopus
2-s2.0-85075214563