Fast UHPLC enantioseparation of liquid crystalline materials with chiral center based on octanol in reversed-phase and polar organic mode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10413777" target="_blank" >RIV/00216208:11310/20:10413777 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/20:00560618

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=xVR6E-9igS" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=xVR6E-9igS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-020-02622-5" target="_blank" >10.1007/s00706-020-02622-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fast UHPLC enantioseparation of liquid crystalline materials with chiral center based on octanol in reversed-phase and polar organic mode

Popis výsledku v původním jazyce

Fast baseline enantioseparation of twenty racemic liquid crystalline compounds was carried out via ultra-performance liquid chromatography through direct enantioseparation in polar organic and reversed-phase modes. Tris(3-chloro-5-methyl-phenylcarbamate) derivative of amylose was used as chiral stationary phase; the chiral selector was covalently immobilized on sub-2 mu m silica particles. Experiments were done using either pure acetonitrile as mobile phase or simple binary mobile phases consisting of acetonitrile/water mixtures in different volume ratios. No buffers or additives were used. Studied materials are a set of structurally similar compounds comprising two different structures of chiral center, various length of alkoxy spacer and four patterns of lateral substitution of aromatic core by fluorine. The compounds do not contain any ionizable group. The effect of the enantiomer structure on the retention and enantioseparation was evaluated. Flow rate and column temperature were optimized as part of method development. Graphic abstract

Název v anglickém jazyce

Fast UHPLC enantioseparation of liquid crystalline materials with chiral center based on octanol in reversed-phase and polar organic mode

Popis výsledku anglicky

Fast baseline enantioseparation of twenty racemic liquid crystalline compounds was carried out via ultra-performance liquid chromatography through direct enantioseparation in polar organic and reversed-phase modes. Tris(3-chloro-5-methyl-phenylcarbamate) derivative of amylose was used as chiral stationary phase; the chiral selector was covalently immobilized on sub-2 mu m silica particles. Experiments were done using either pure acetonitrile as mobile phase or simple binary mobile phases consisting of acetonitrile/water mixtures in different volume ratios. No buffers or additives were used. Studied materials are a set of structurally similar compounds comprising two different structures of chiral center, various length of alkoxy spacer and four patterns of lateral substitution of aromatic core by fluorine. The compounds do not contain any ionizable group. The effect of the enantiomer structure on the retention and enantioseparation was evaluated. Flow rate and column temperature were optimized as part of method development. Graphic abstract

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-19655S" target="_blank" >GA20-19655S: Strategie pro on-line štěpení proteinů s následnou separací v mix-mode chromatografii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Svazek periodika

151

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

6

Strana od-do

1235-1240

Kód UT WoS článku

000555979300008

EID výsledku v databázi Scopus

2-s2.0-85085570057