The evolution of the Puf superfamily of proteins across the tree of eukaryotes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10413787" target="_blank" >RIV/00216208:11310/20:10413787 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bl_18nY4Vr" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bl_18nY4Vr</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12915-020-00814-3" target="_blank" >10.1186/s12915-020-00814-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The evolution of the Puf superfamily of proteins across the tree of eukaryotes

Popis výsledku v původním jazyce

Background Eukaryotic gene expression is controlled by a number of RNA-binding proteins (RBP), such as the proteins from the Puf (Pumilio and FBF) superfamily (PufSF). These proteins bind to RNA via multiple Puf repeat domains, each of which specifically recognizes a single RNA base. Recently, three diversified PufSF proteins have been described in model organisms, each of which is responsible for the maturation of ribosomal RNA or the translational regulation of mRNAs; however, less is known about the role of these proteins across eukaryotic diversity. Results Here, we investigated the distribution and function of PufSF RBPs in the tree of eukaryotes. We determined that the following PufSF proteins are universally conserved across eukaryotes and can be broadly classified into three groups: (i) Nop9 orthologues, which participate in the nucleolar processing of immature 18S rRNA; (ii) &apos;classical&apos; Pufs, which control the translation of mRNA; and (iii) PUM3 orthologues, which are involved in the maturation of 7S rRNA. In nearly all eukaryotes, the rRNA maturation proteins, Nop9 and PUM3, are retained as a single copy, while mRNA effectors (&apos;classical&apos; Pufs) underwent multiple lineage-specific expansions. We propose that the variation in number of &apos;classical&apos; Pufs relates to the size of the transcriptome and thus the potential mRNA targets. We further distinguished full set of PufSF proteins in divergent metamonadGiardia intestinalisand initiated their cellular and biochemical characterization. Conclusions Our data suggest that the last eukaryotic common ancestor (LECA) already contained all three types of PufSF proteins and that &apos;classical&apos; Pufs then underwent lineage-specific expansions.

Název v anglickém jazyce

The evolution of the Puf superfamily of proteins across the tree of eukaryotes

Popis výsledku anglicky

Background Eukaryotic gene expression is controlled by a number of RNA-binding proteins (RBP), such as the proteins from the Puf (Pumilio and FBF) superfamily (PufSF). These proteins bind to RNA via multiple Puf repeat domains, each of which specifically recognizes a single RNA base. Recently, three diversified PufSF proteins have been described in model organisms, each of which is responsible for the maturation of ribosomal RNA or the translational regulation of mRNAs; however, less is known about the role of these proteins across eukaryotic diversity. Results Here, we investigated the distribution and function of PufSF RBPs in the tree of eukaryotes. We determined that the following PufSF proteins are universally conserved across eukaryotes and can be broadly classified into three groups: (i) Nop9 orthologues, which participate in the nucleolar processing of immature 18S rRNA; (ii) &apos;classical&apos; Pufs, which control the translation of mRNA; and (iii) PUM3 orthologues, which are involved in the maturation of 7S rRNA. In nearly all eukaryotes, the rRNA maturation proteins, Nop9 and PUM3, are retained as a single copy, while mRNA effectors (&apos;classical&apos; Pufs) underwent multiple lineage-specific expansions. We propose that the variation in number of &apos;classical&apos; Pufs relates to the size of the transcriptome and thus the potential mRNA targets. We further distinguished full set of PufSF proteins in divergent metamonadGiardia intestinalisand initiated their cellular and biochemical characterization. Conclusions Our data suggest that the last eukaryotic common ancestor (LECA) already contained all three types of PufSF proteins and that &apos;classical&apos; Pufs then underwent lineage-specific expansions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Biology

ISSN

1741-7007

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

77

Kód UT WoS článku

000546932800001

EID výsledku v databázi Scopus

2-s2.0-85087409692