The effect of particle and ligand types on retention and peak shape in liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10416654" target="_blank" >RIV/00216208:11310/20:10416654 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=z_IygrEP2v" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=z_IygrEP2v</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2020.105466" target="_blank" >10.1016/j.microc.2020.105466</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of particle and ligand types on retention and peak shape in liquid chromatography

Popis výsledku v původním jazyce

A well performed, detailed characterization of stationary phase properties can serve as a useful tool for choice of suitable separation environment for a given purpose. This work shows effects of particle type/technology and ligand chemistry on retention and peak shape of neutral, acidic, basic and zwitterionic compounds. Eight columns differing in endcapping technology, particle type and ligand bonded were evaluated, namely XSelect CSH C18, XSelect CSH Phenyl-Hexyl, XSelect CSH Fluoro-Phenyl, XSelect HSS C18, XSelect HSS C18 SB, XSelect HSS PFP, XBridge C18 and XBridge Shield RP18. A simple Walters test for evaluation of hydrophobicity and silanol activity was performed. Mobile phases composed of acetonitrile and aqueous part (pH 2.1; pH 4.7 and pH 8.0) were used. More detailed description of interactions participating in the retention mechanism was provided by the linear free energy relationship model. For a more detailed characterization of possible electrostatic interactions, compounds with permanent charge across selected pH range were used. A good agreement between the Walters test results, the expanded linear free energy relationship model, and the test of ionic-interactions was observed, suggesting that each testing method provides value for characterization of the solute-stationary phase interactions.

Název v anglickém jazyce

The effect of particle and ligand types on retention and peak shape in liquid chromatography

Popis výsledku anglicky

A well performed, detailed characterization of stationary phase properties can serve as a useful tool for choice of suitable separation environment for a given purpose. This work shows effects of particle type/technology and ligand chemistry on retention and peak shape of neutral, acidic, basic and zwitterionic compounds. Eight columns differing in endcapping technology, particle type and ligand bonded were evaluated, namely XSelect CSH C18, XSelect CSH Phenyl-Hexyl, XSelect CSH Fluoro-Phenyl, XSelect HSS C18, XSelect HSS C18 SB, XSelect HSS PFP, XBridge C18 and XBridge Shield RP18. A simple Walters test for evaluation of hydrophobicity and silanol activity was performed. Mobile phases composed of acetonitrile and aqueous part (pH 2.1; pH 4.7 and pH 8.0) were used. More detailed description of interactions participating in the retention mechanism was provided by the linear free energy relationship model. For a more detailed characterization of possible electrostatic interactions, compounds with permanent charge across selected pH range were used. A good agreement between the Walters test results, the expanded linear free energy relationship model, and the test of ionic-interactions was observed, suggesting that each testing method provides value for characterization of the solute-stationary phase interactions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-19655S" target="_blank" >GA20-19655S: Strategie pro on-line štěpení proteinů s následnou separací v mix-mode chromatografii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

—

Svazek periodika

159

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

105466

Kód UT WoS článku

000598759800007

EID výsledku v databázi Scopus

2-s2.0-85090131928