How ligand and particle types affect retention and separation of structurally diverse biologically active compounds in HPLC
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10431244" target="_blank" >RIV/00216208:11310/21:10431244 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=IoiiCE9dS2" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=IoiiCE9dS2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-021-02807-6" target="_blank" >10.1007/s00706-021-02807-6</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
How ligand and particle types affect retention and separation of structurally diverse biologically active compounds in HPLC
Popis výsledku v původním jazyce
In this work, effects of particle and ligand types on retention and separation of sets of neutral (naphthoflavones), acidic (profens), basic (cathinones), and zwitterionic (dipeptides) biologically active compounds are shown in detail and discussed. Nine columns differing in ligand and particle types were tested, namely XSelect HSS C18, XSelect HSS C18 SB, XSelect HSS PFP, XSelect CSH C18, XSelect CSH Phenyl-Hexyl, XSelect CSH Fluoro-Phenyl, XBridge C18, XBridge Shield RP18, and Atlantis PREMIER BEH C18 AX. Various mobile phases differing in acetonitrile/aqueous part volume ratio and aqueous part pH, i.e. pH = 2.1, pH = 4.7, and pH = 8.0 were tested and compared in terms of analytes ' retention and separation. Results showed significant effect of ionic interactions resulting from particle types used or additional ionisable functional groups bonded to the surface on retention and separation of charged compounds. All tested sets of compounds were baseline separated but different conditions, that is column type and mobile phase composition, were needed.
Název v anglickém jazyce
How ligand and particle types affect retention and separation of structurally diverse biologically active compounds in HPLC
Popis výsledku anglicky
In this work, effects of particle and ligand types on retention and separation of sets of neutral (naphthoflavones), acidic (profens), basic (cathinones), and zwitterionic (dipeptides) biologically active compounds are shown in detail and discussed. Nine columns differing in ligand and particle types were tested, namely XSelect HSS C18, XSelect HSS C18 SB, XSelect HSS PFP, XSelect CSH C18, XSelect CSH Phenyl-Hexyl, XSelect CSH Fluoro-Phenyl, XBridge C18, XBridge Shield RP18, and Atlantis PREMIER BEH C18 AX. Various mobile phases differing in acetonitrile/aqueous part volume ratio and aqueous part pH, i.e. pH = 2.1, pH = 4.7, and pH = 8.0 were tested and compared in terms of analytes ' retention and separation. Results showed significant effect of ionic interactions resulting from particle types used or additional ionisable functional groups bonded to the surface on retention and separation of charged compounds. All tested sets of compounds were baseline separated but different conditions, that is column type and mobile phase composition, were needed.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10403 - Physical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GA20-19655S" target="_blank" >GA20-19655S: Strategie pro on-line štěpení proteinů s následnou separací v mix-mode chromatografii</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Monatshefte für Chemie - Chemical Monthly
ISSN
0026-9247
e-ISSN
—
Svazek periodika
152
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
AT - Rakouská republika
Počet stran výsledku
8
Strana od-do
1081-1088
Kód UT WoS článku
000685590000001
EID výsledku v databázi Scopus
2-s2.0-85112775063