The dynamics of band (peak) shape development in capillary zone electrophoresis in the case of two co-migrating analytes: The displacement and the tag-along effects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10419869" target="_blank" >RIV/00216208:11310/20:10419869 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=DmY5kxTlJN" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=DmY5kxTlJN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201900225" target="_blank" >10.1002/elps.201900225</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The dynamics of band (peak) shape development in capillary zone electrophoresis in the case of two co-migrating analytes: The displacement and the tag-along effects

Popis výsledku v původním jazyce

Peak shapes in electrophoresis are often distorted from the ideal Gaussian shape due to disturbing phenomena, of which the most important is electromigration dispersion. For fully dissociated analytes, there is a tight analogy between nonlinear models describing a separation process in chromatography and electrophoresis. When the velocity of the separated analyte depends on the concentration of the co-analyte, the consequence is a mutual influence of the analytes couples, which distorts both analyte zones. In this paper, we introduce a nonlinear model of electromigration for the analysis of two co-migrating fully dissociated analytes. In the initial stages of separation, they influence each other, which causes much more complicated peak shapes. The analysis has revealed that the two most important phenomena-the displacement and the tag-along effects-are common both for nonlinear chromatography and electrophoresis, though their description is partly based on rather different phenomena. The comparison between the nonlinear model of electromigration we describe and the numerical computer solution of the original continuity equations has proven an almost perfect agreement. The predicted features in peak shapes in initial stages of separation have been fully confirmed by the experiments.

Název v anglickém jazyce

The dynamics of band (peak) shape development in capillary zone electrophoresis in the case of two co-migrating analytes: The displacement and the tag-along effects

Popis výsledku anglicky

Peak shapes in electrophoresis are often distorted from the ideal Gaussian shape due to disturbing phenomena, of which the most important is electromigration dispersion. For fully dissociated analytes, there is a tight analogy between nonlinear models describing a separation process in chromatography and electrophoresis. When the velocity of the separated analyte depends on the concentration of the co-analyte, the consequence is a mutual influence of the analytes couples, which distorts both analyte zones. In this paper, we introduce a nonlinear model of electromigration for the analysis of two co-migrating fully dissociated analytes. In the initial stages of separation, they influence each other, which causes much more complicated peak shapes. The analysis has revealed that the two most important phenomena-the displacement and the tag-along effects-are common both for nonlinear chromatography and electrophoresis, though their description is partly based on rather different phenomena. The comparison between the nonlinear model of electromigration we describe and the numerical computer solution of the original continuity equations has proven an almost perfect agreement. The predicted features in peak shapes in initial stages of separation have been fully confirmed by the experiments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA18-11776S" target="_blank" >GA18-11776S: Pokročilé teoretické a softwarové nástroje pro elektroforézu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

481-492

Kód UT WoS článku

000491680700001

EID výsledku v databázi Scopus

2-s2.0-85074589886