Canagliflozin oxidation study using electrochemical flow cell and comparison with hydrogen peroxide oxidation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10420313" target="_blank" >RIV/00216208:11310/20:10420313 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc16.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc16.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Canagliflozin oxidation study using electrochemical flow cell and comparison with hydrogen peroxide oxidation

Popis výsledku v původním jazyce

By standards, the effect on oxidation of an active substance is tested using hydrogen peroxide solution at elevated temperature in a stress chamber for 1-7 days. An alternative way to study the effect of oxidation on an active substance is to use an electrochemical flow cell. Solution with active substance flows at low flow rate into a small reactor, where the active substance is oxidized on working electrode surface. The electrolyte stream with the oxidized active substance is then directed to the sample collector. Products of electrochemical oxidation are analyzed by high performance liquid chromatography with ultraviolet-visible spectrophotometry detection. Canagliflozin has been used because its main degradation pathway is oxidation. The design of experiments approach was used to explore the experimental space and optimize experimental conditions of oxidation. The results of the oxidation study performed in the electrochemical flow cell were statistically compared with the results of a standard study using hydrogen peroxide solution. The most suitable conditions for electrochemical oxidation were found. Electrochemical oxidation produced comparable amounts of impurities as chemical oxidation with hydrogen peroxide.

Název v anglickém jazyce

Canagliflozin oxidation study using electrochemical flow cell and comparison with hydrogen peroxide oxidation

Popis výsledku anglicky

By standards, the effect on oxidation of an active substance is tested using hydrogen peroxide solution at elevated temperature in a stress chamber for 1-7 days. An alternative way to study the effect of oxidation on an active substance is to use an electrochemical flow cell. Solution with active substance flows at low flow rate into a small reactor, where the active substance is oxidized on working electrode surface. The electrolyte stream with the oxidized active substance is then directed to the sample collector. Products of electrochemical oxidation are analyzed by high performance liquid chromatography with ultraviolet-visible spectrophotometry detection. Canagliflozin has been used because its main degradation pathway is oxidation. The design of experiments approach was used to explore the experimental space and optimize experimental conditions of oxidation. The results of the oxidation study performed in the electrochemical flow cell were statistically compared with the results of a standard study using hydrogen peroxide solution. The most suitable conditions for electrochemical oxidation were found. Electrochemical oxidation produced comparable amounts of impurities as chemical oxidation with hydrogen peroxide.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 16th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-079-3

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

76-82

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

17. 9. 2020

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000623101400014