Spectrometric determination of trimethoprim and sulfamethoxazole in tablets by automated sequential injection technique

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10423509" target="_blank" >RIV/00216208:11310/20:10423509 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ESfs0p3Ryl" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ESfs0p3Ryl</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-020-02631-4" target="_blank" >10.1007/s00706-020-02631-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spectrometric determination of trimethoprim and sulfamethoxazole in tablets by automated sequential injection technique

Popis výsledku v původním jazyce

An automated sequential injection analysis with spectrometric detection for the determination of trimethoprim and sulfamethoxazole in tablets is proposed. The concentration of sulfamethoxazole is determined by monitoring the absorbance at 540 nm due to the intensively colored complex formed by derivatization reaction of sulfamethoxazole with nitrite and N-(1-naphthyl)ethylenediamine in acidic media. With knowledge of the sulfamethoxazole concentration, the trimethoprim concentration can be calculated from the absorption of the sample solution at 264 nm, where both analytes absorb, and the additivity of individual absorbances according to Beer-Lambert law can be used. The developed method has a linear dynamic range of 0.03-5.0 mg dm(-3) and a detection limit of 0.03 mg dm(-3) for trimethoprim and a linear dynamic range of 0.1-10.0 mg dm(-3) and a detection limit of 0.01 mg dm(-3) for sulfamethoxazole. The sampling frequency of the method is 24 h(-1). The method was successfully applied on pharmaceutical dosage form of tablets, as the results of the determination are in a good agreement with the official pharmacopoeial method (HPLC). The developed sequential injection analysis is simpler, cheaper, and faster than already published methods and pharmacopoeial assay. So it appears to be suitable for quality control in the pharmaceutical industry.

Název v anglickém jazyce

Spectrometric determination of trimethoprim and sulfamethoxazole in tablets by automated sequential injection technique

Popis výsledku anglicky

An automated sequential injection analysis with spectrometric detection for the determination of trimethoprim and sulfamethoxazole in tablets is proposed. The concentration of sulfamethoxazole is determined by monitoring the absorbance at 540 nm due to the intensively colored complex formed by derivatization reaction of sulfamethoxazole with nitrite and N-(1-naphthyl)ethylenediamine in acidic media. With knowledge of the sulfamethoxazole concentration, the trimethoprim concentration can be calculated from the absorption of the sample solution at 264 nm, where both analytes absorb, and the additivity of individual absorbances according to Beer-Lambert law can be used. The developed method has a linear dynamic range of 0.03-5.0 mg dm(-3) and a detection limit of 0.03 mg dm(-3) for trimethoprim and a linear dynamic range of 0.1-10.0 mg dm(-3) and a detection limit of 0.01 mg dm(-3) for sulfamethoxazole. The sampling frequency of the method is 24 h(-1). The method was successfully applied on pharmaceutical dosage form of tablets, as the results of the determination are in a good agreement with the official pharmacopoeial method (HPLC). The developed sequential injection analysis is simpler, cheaper, and faster than already published methods and pharmacopoeial assay. So it appears to be suitable for quality control in the pharmaceutical industry.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Svazek periodika

151

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

6

Strana od-do

1311-1316

Kód UT WoS článku

000537679200003

EID výsledku v databázi Scopus

2-s2.0-85085975314