Sensitive fluorescence in situ hybridization on semithin sections of adult Schistosoma mansoni using DIG-labeled RNA probes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10448302" target="_blank" >RIV/00216208:11310/20:10448302 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/20:00525099

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-0635-3_4" target="_blank" >https://doi.org/10.1007/978-1-0716-0635-3_4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-0635-3_4" target="_blank" >10.1007/978-1-0716-0635-3_4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sensitive fluorescence in situ hybridization on semithin sections of adult Schistosoma mansoni using DIG-labeled RNA probes

Popis výsledku v původním jazyce

In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms; however, it can be successfully applied to other trematode species.

Název v anglickém jazyce

Sensitive fluorescence in situ hybridization on semithin sections of adult Schistosoma mansoni using DIG-labeled RNA probes

Popis výsledku anglicky

In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms; however, it can be successfully applied to other trematode species.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Schistosoma mansoni: Methods and Protocols

ISBN

978-1-07-160634-6

Počet stran výsledku

11

Strana od-do

43-53

Počet stran knihy

265

Název nakladatele

Springer, Humana Press NY

Místo vydání

New York, United States

Kód UT WoS kapitoly

—